Effects of Sample Injection Volume

Effects of sample injection volume when Asahipak NH2P-50 4E is used is shown. When making sample solution to be injected, sample should be dissolved in a solvent whose composition is as close as the eluent, preferably in the eluent. When 75% acetonitrile aqueous solution is used for the eluent and a sample can be dissolved only in aqueous solution (or 50v/v% ethanol, etc.), injection volume should be as small as possible.


Sample : 20μg each in purified water
1. Fructose 2. Glucose 3. Sucrose 4. Lactose 5. Maltose

Column       : Shodex Asahipak NH2P-50 4E (4.6mmI.D. x 250mm)
Eluent       : CH3CN/H2O=75/25
Flow rate    : 1.0mL/min
Detector     : Shodex RI
Column temp. : 25°C