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Effects of Temperature (1)


Effects of temperature when analyzing saccharides using SUGAR SC1011 (a column for saccharides analysis) is shown here. Saccharides having carbonyl or aldehyde group may have anomers. It is possible to prevent anomer separation by analyzing at elavated temperature. It is also possible to have higher theoretical plate number and resolution by it. Analyzing at elevated temperature around 80 °C is recommended when using columns such as SP0810, SC1011, SC1821, KS-801 and KS-802.

Sample : 0.5%each, 5μL
1. Isomaltotriose
2. Sucrose
3. Glucose
4. Lactitol
5. Fructose
6. meso-Erythritol
7. Mannitol
8. Dulcitol
9. Xylitol

Column    : Shodex SUGAR SC1011 (8.0mmI.D. x 300mm)
Eluent    : H2O
Flow rate : 0.6mL/min
Detector  : Shodex RI

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