Effects of Temperature (2)

Raffinose was separated using SUGAR SH1011. Since raffinose can be easily hydrolyzed by acids, it should be analyzed at ordinary temperature. The situation is the same for other saccharides such as sucrose.

Sample : Raffinose

Column       : Shodex SUGAR SH1011 (8.0 mm I.D. x 300 mm)
Eluent       : 5 mM H2SO4 aq.
Flow rate    : 0.6 mL/min
Detector     : RI
Column temp. : (above); 60 °C, (below); 25 °C

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