When analyzing saccharides in food, it is necessary to remove the substances besides saccharides before the analysis. This procedure is called as sample pre-treatment. Here, the analysis result of saccharides in food using Asahipak NH2P-50 4E is shown together with the sample pre-treatment procedure.

(Sample pre-treatment for jelly)
1) Put 5 g of jelly in a beaker and add 30 mL of 50 %(V/V) ethanol.
2) Extract the substances using an ultrasonic vibrator for 30 minutes.
3) Put the paste and ethanol in a 50 mL volumetric flask and add 50 %(V/V) ethanol to make up a 50 mL solution.
4) Filtrate the solution with a paper filter (No.5B).
5) Take a part of filtrated solution and add the same volume of acetonitrile.
6) Pass through a 0.45 μm membrane filter before injecting into HPLC.

Sample : Jelly, 20 μL
1. Fructose
2. Glucose
3. Sucrose

Column       : Shodex Asahipak NH2P-50 4E (4.6 mm I.D. x 250 mm)
Eluent       : CH3CN/H2O=75/25
Flow rate    : 1.0 mL/min
Detector     : RI
Column temp. : Room temp. (25 °C)

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