Five kinds of aldose were analyzed at high recovery ratio by HILICpak VG-50 4E, a polymer-based amino column. VG-50 is suitable for high sensitive analysis of reducing sugars, like aldose.
Sample : 10 μL
2 mg/mL each
(in CH3CN/H2O=50/50)
1. Arabinose
2. Xylose
3. Galactose
4. Mannose
5. Glucose
Column : Shodex HILICpak VG-50 4E (4.6 mm I.D. x 250 mm) Eluent : CH3CN/8 mM NaOH + 5 mM NaCl aq.=82/18 Flow rate : 1.0 mL/min Detector : RI Column temp. : 40 °C
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