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Analysis of Aldoses (VG-50 4E)


Five kinds of aldose were analyzed at high recovery ratio by HILICpak VG-50 4E, a polymer-based amino column. VG-50 is suitable for high sensitive analysis of reducing sugars, like aldose.

Sample : 10μL
2mg/mL each
(in CH3CN/H2O=50/50)
1. Arabinose
2. Xylose
3. Galactose
4. Mannose
5. Glucose

Column       : Shodex HILICpak VG-50 4E (4.6mmI.D. x 250mm)
Eluent       : CH3CN/8mM NaOH + 5mM NaCl aq.=82/18
Flow rate    : 1.0mL/min
Detector     : Shodex RI
Column temp. : 40°C

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