According to the United States Pharmacopeia and the National Formulary (USP 39-NF 34*), ”ASSAY" and "IMPURITIES: Related Compounds" for "Ribose" should be carried out with a column filled with L22 packing material. The SUGAR KS-801, a column filled with L22 packing material, confirmed the requirements were met.

System suitability requirementsResolution between ribose and arabinose (System suitability solution): ≥ 1.2
Tailing factor (Standard solution): ≥ 1.5
Theoretical plate number of ribose (Standard solution): ≥ 2,500
Relative standard deviation (RSD) (Standard solution): ≤ 2.0%

*The version at the time of the application acquisition.

Sample: 10 µL
(System suitability solution) 20 mg/mL of Ribose and 0.2 mg/mL of  Arabinose in eluent

1. 1.Arabinose
2. 2.Ribose