According to the United States Pharmacopeia and the National Formulary (USP 39-NF 34*), analysis of ribose should be carried out with a column with L22 packing material and meets following requirements. The SUGAR KS-801 confirmed the requirements were met.
System suitability requirements:
Resolution of ribose and arabinose: ≥ 1.2
Tailing factor: ≤ 1,5
Theoretical plate number: ≥ 2,500
Relative standard deviation (RSD): ≤ 2.0 %
*The version at the time of the application acquisition.
Sample : 10 µL
1. Arabinose 0.02 %
2. Ribose 2 %
Column : Shodex SUGAR KS-801 (8.0 mm I.D. x 300 mm) Eluent : H2O Flow rate : 1.0 mL/min Detector : RI Column temp. : 80 °C
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