Analysis of Ribose According to USP-NF Method (KS-801)

According to the United States Pharmacopeia and the National Formulary (USP 39-NF 34*), analysis of ribose should be carried out with a column with L22 packing material and meets following requirements. The SUGAR KS-801 confirmed the requirements were met.

System suitability requirements

Resolution of ribose and arabinose: ≥ 1.2
Tailing factor: ≤ 1,5
Theoretical plate number: ≥ 2,500
Relative standard deviation (RSD): ≤ 2.0%

Sample: 10 µL

  1. 1.Arabinose 0.02 %
  2. 2.Ribose 2 %
Column
Shodex SUGAR KS-801 (8.0 mm I.D. x 300 mm)
Eluent
H2O
Flow rate
1.0 mL/min
Detector
RI
Column temp.
80 ℃

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