Analysis of Ribose According to USP-NF Method (KS-801)

According to the United States Pharmacopeia and the National Formulary (USP 39-NF 34*), analysis of ribose should be carried out with a column with L22 packing material and meets following requirements. The SUGAR KS-801 confirmed the requirements were met.

System suitability requirements:
   Resolution of ribose and arabinose: ≥ 1.2
   Tailing factor: ≤ 1,5 
   Theoretical plate number: ≥ 2,500
   Relative standard deviation (RSD): ≤ 2.0%

*The version at the time of the application acquisition.


Sample : 10 µL
1. Arabinose 0.02 %
2. Ribose 2 %

Column       : Shodex SUGAR KS-801 (8.0 mm I.D. x 300 mm)
Eluent       : H2O
Flow rate    : 1.0 mL/min
Detector     : Shodex RI
Column temp. : 80 °C