Analysis of Dextrose and Fructose in Cranberry Juice (NH2P-50 4E)

According to the United States Pharmacopeia and the National Formulary monograph for cranberry juice (USPNF 2022 Issue 3*) uses a silica-based amino column for the analysis of dextrose (D-glucose) and fructose. In this application, we used a polymer-based amino column, Asahiak NH2P-50 4E, and referred to the USP monograph method. The results showed that NH2P-50 4E is also feasible to analyze dextrose and fructose in cranberry juice.

* The version at the time of the application acquisition.

Sample pretreatment

  1. (1)Filter cranberry juice with a 0.45-μm filter membrane and use it as an injection sample.

Sample: 10 µL each
Stadard solution (Fructose, Dextrose 30 mg/mL each)
100% Cranberry fruit juice

  1. 1.Fructose
  2. 2.Dextrose(D-Glucose)
chromatogram of fructose and dextrose in cranberry fruit juice
Shodex Asahipak NH2P-50 4E (4.6 mm I.D. x 250 mm)
Flow rate
1.0 mL/min
Column temp.
30 ℃

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