Monosaccharides and Disaccharides were analyzed by HILICpak VG-50 4E, a polymer-based amino column. The recovery ratio of reducing sugars by VG-50 is high, so the peak of galactose can be detected higher than by the former amino columns. VG-50 is suitable for high sensitive analysis.
Sample : 0.05 % each, 10 μL
1. Fructose
2. Galactose
3. Glucose
4. Sucrose
5. Lactose
6. Maltose
Column : Shodex HILICpak VG-50 4E (4.6 mm I.D. x 250 mm) Eluent : CH3CN/CH3OH/H2O=85/5/10 Flow rate : 1.0 mL/min Detector : Corona charged aerosol Column temp. : 60 °C
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