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Analysis of Tagatose According to USP-NF Method (SC1011)


According to the United States Pharmacopeia and the National Formulary (USP 40-NF 35*), the assay of tagatose should be carried out with a column with L22 packing material and meets following requirements. The SUGAR SC1011 confirmed the requirements were met.

System suitability requirements:
    Relative standard deviation (RSD): ≤ 2.0%

*The version at the time of the application acquisition.

Sample : 20 µL
1. Tagatose 5 mg/mL

Column       : Shodex SUGAR SC1011 (8.0 mm I.D. x 300 mm)
Eluent       : 0.05 mg/mL Calcium acetate aq.
Flow rate    : 0.6 mL/min
Detector     : Shodex RI
Column temp. : 85 °C

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