Japanese Notification Eishin No. 47* (May 23, 1996, Ministry of Health, Labour and Welfare (MHLW)) “Analytical Methods for Nutritional Components, Following the Introduction of Nutrition Labeling Standards” includes “Appendix: Analytical Methods for Nutritional Components”. Under section 7. Sugars, monosaccharides or disaccharides that are not sugar alcohols are classified as sugars. (2) High-Performance Liquid Chromatography Method is divided into two parts. 1) Monosaccharides, Disaccharides, and Oligosaccharides and 2) Sugar Alcohols. Under 1) Monosaccharides, Disaccharides, and Oligosaccharides, columns packed with silica gel modified with aminopropyl groups (polymer-based amino columns are also acceptable) are used. In this application, Asahipak NH2P-50 4E, a polymer-based amino column, was used to analyze standards of common monosaccharides and disaccharides contained in foods. A mixed solvent of water and acetonitrile was used as the mobile phase, and by adjusting the mixing ratio, the elution of the sample could be controlled. Polymer-based amino columns exhibit less change over time compared to silica-based amino columns and can also be cleaned with alkaline solutions.

*The version at the time of the application acquisition.

Sample: 20 µL
0.1 % each

1. 1.Fructose
2. 2.Sorbose
3. 3.Galactose
4. 4.Glucose
5. 5.Xylobiose
6. 6.Sucrose
7. 7.Lactose
8. 8.Maltose