Effects of Acetonitrile Concentration on Elution Time (NH2P-50 4E)

The separation of saccharides using Asahipak NH2P-50 column can be controlled by changing mixing ratio of acetonitrile and water in the eluent. Since the NH2P-50 column is free from non-specific adsorption, sharp and symmetrical peaks can be obtained for most of saccharides.
When the NH2P-50 column is used, as same as when silica-based amino columns are used, the retention time of saccharides becomes longer as the acetonitrile concentration in the eluent becomes higher. The elution order of saccharides using the NH2P-50 columns is almost as same as that using silica-based amino columns though there are some exception such as the order between galactose and glucose and between lactose and maltose.

Sample :
1. Rhamnose
2. Xylose
3. Fructose
4. Glucose
5. Sucrose
6. Maltose
7. Lactose
8. Galactose
9. Raffinose
10. Mannose

Columns      : Shodex Asahipak NH2P-50 4E (4.6 mm I.D. x 250 mm) ,
               Silica based amino column(other manufacturer)
Eluent       : CH3CN/H2O
Flow rate    : 1.0 mL/min
Detector     : RI
Column temp. : 30 °C

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