Saccharides in chocolate cake were analyzed using Asahipak NH2P-50 4E (a column for saccharides analysis).

Sample pretreatment

1. 1.Take 5 g of chocolate cake in a vial for centrifuge.
2. 2.Add 30 mL of petroleum ether and stir it for 15 minutes.
3. 3.Then use centrifuge for 10 minutes at 2,000 rpm and take off the supernatant.
4. 4.Heat the sample solution to remove petroleum ether in the water bath at 40 ℃.
5. 5.Add 30 mL of the mixture of 50 % (V/V) ethanol solution.
6. 6.Apply ultrasonic vibration for 30 minutes a make 50 mL of solution with the mixture of 50 % (V/V) ethanol.
7. 7.Filtrate with No.5B paper filter.
8. 8.Take 3 mL of filtrated sample and add 3 mL of acetonitrile.
9. 9.Filtrate with 0.45 μm membrane filter.

Sample: 20 μL
Chocolate cake