Saccharides Analysis using a Charged Aerosol Detector (NH2P-50 4E)

Since charged aerosol detectors (CAD) measure analytes as particles, components eluting from columns can significantly affect the baseline. A polymer-based amino column Asahipak NH2P-50 series releases a very few components from the column, resulting in a low noise level and a stable baseline.

Sample: 5 µL
40 µg/mL each

  1. 1.Fructose
  2. 2.Glucose
Column
：Shodex Asahipak NH2P-50 4E (4.6 mm I.D. x 250 mm)
Silica based amino column from other manufacturer (4.6 mm I.D. x 250 mm)
Silica based amide column from other manufacturer (4.6 mm I.D. x 150 mm)
Eluent
：H2O/CH3CN=20/80
Flow rate
：1.0 mL/min
Detector
：Charged aerosol
Column temp.
：30 ℃ (Amino column), 80 ℃ (Amide column)

Since corona charged aerosol detector (CAD) measures components of elute as well as the analyte, anything elute from column influences the baseline largely. The polymer-based amino column, Asahipak NH2P-50 4E has very small "bleeding" and thus provides stable baseline without much noise compared to silica-based columns.


Sample : 40 μg/mL each, 5 μL
1. Fructose
2. Glucose

  
Columns      : Shodex Asahipak NH2P-50 4E (4.6 mm I.D. x 250 mm)
               Silica based amino column from other manufacturer (4.6 mm I.D. x 250 mm)
               Silica based amide column from other manufacturer (4.6 mm I.D. x 150 mm)
Eluent       : H2O/CH3CN=20/80
Flow rate    : 1.0 mL/min
Detector     : Corona charged aerosol
Column temp. : 30 °C (Amino column), 80 °C (Amide column)

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