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Saccharides Analysis using Corona Charged Aerosol Detector

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Since corona charged aerosol detector (CAD) measures components of elute as well as the analyte, anything elute from column influences the baseline largely. The polymer-based amino column, Asahipak NH2P-50 4E has very small "bleeding" and thus provides stable baseline without much noise compared to silica-based columns.


Sample : 40μg/mL each, 5μL
1. Fructose
2. Glucose


Columns      : Shodex Asahipak NH2P-50 4E (4.6mmI.D. x 250mm)
               Silica based amino column from other manufacturer (4.6mmI.D. x 250mm)
               Silica based amide column from other manufacturer (4.6mmI.D. x 150mm)
Eluent       : H2O/CH3CN=20/80
Flow rate    : 1.0mL/min
Detector     : Corona charged aerosol
Column temp. : 30°C (Amino column), 80°C (Amide column)

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