Below table summarizes elution volumes of saccharides using seven different Shodex columns for sugar analyses: SUGAR SP0810, SC1011. KS-801, SZ5532, and SC1211, RSpak DC-613, Asahipak NH2P-50 4E, and GS-220 HQ. Elution values shown are the peak top positions, please contact us to request a chromatogram if you would like to see their elution patterns.
Click here for a list of “Elution Volume of Saccharides” using HILICpak VG-50 4E under different analytical conditions.
Click here for a list of “Elution Volume of Saccharides” using HILICpak VG-25 4D.
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| Substance | Elution Volume (mL) | |||||||
|---|---|---|---|---|---|---|---|---|
|
SP0810 |
SC1011 |
KS-801 |
SZ5532 |
SC1211 |
DC-613 |
NH2P-50 4E |
VG-50 4E |
|
| N-Acetyl-α-D-glucosamine | 8.86 | 7.75 | 6.68 | 2 peaks | 4.10 | 2 peaks | 6.66 | N/A |
| D(+)-Arabinose | 10.42 | 8.91 | 8.21 | 5.11 | 5.56 | 5.75 | 6.18 | 5.65 |
| D-Arabitol | 15.86 | 11.33 | 7.63 | 7.27 | 8.16 | 5.81 | 6.29 | 6.05 |
| Aspartame | － | － | － | － | 34.02 | － | － | N/A |
| 2-Deoxy-D-glucose | 8.83 | 7.58 | 7.15 | 4.34 | 4.02 | 4.43 | 6.02 | N/A |
| Difructose anhydride III | 7.07 | 6.30 | 5.81 | 4.30 | * | 4.51 | 7.77 | N/A |
| Dulcitol | 20.18 | 12.76 | 7.40 | 9.46 | 11.28 | 7.33 | 7.45 | N/A |
| meso-Erythritol | 12.70 | 10.09 | 7.86 | 5.73 | 6.27 | 4.84 | 5.43 | 5.10 |
| Ethanol | 11.13 | 11.33 | 10.09 | － | 4.27 | － | － | N/A |
| 1-Fructofuranosyl-D-nystose | 6.05 | 5.27 | 4.76 | 31.43 | * | － | － | N/A |
| D(-)-Fructose | 11.05 | 8.85 | 7.71 | 5.37 | 5.90 | 6.19 | 6.75 | 6.19 |
| D(+)-Fucose | 10.48 | 8.84 | 8.09 | 4.50 | 4.96 | 4.81 | 5.43 | 5.06 |
| D(+)-Galactose | 9.74 | 7.98 | 7.58 | 6.46 | 4.98 | 7.28 | 8.10 | 7.37 |
| 4'-Galactosyllactose | 7.42 | 6.02 | 5.40 | 19.02 | * | 21.24 | 21.66 | N/A |
| α-D-Galacturonic acid | － | 6.28 | 4.36 | － | 5.63 | － | － | N/A |
| Gentiobiose | 7.22 | 6.08 | 5.75 | 10.50 | * | 13.18 | 16.36 | 14.52 |
| Glucose | 8.63 | 7.30 | 7.17 | 5.87 | 4.76 | 6.83 | 8.61 | 7.47 |
| Glycerol | N/A | N/A | N/A | N/A | N/A | 4.26 | N/A | N/A |
| Glycyrrhizin | － | － | － | － | 2.71 | 2.00 | － | N/A |
| myo-Inositol | 12.77 | 8.86 | 7.99 | 12.63 | 7.87 | 15.80 | 9.96 | 11.68 |
| Isomaltose | 7.68 | 6.26 | 5.95 | 10.57 | * | 13.82 | 15.18 | 14.41 |
| Isomaltotriose | 7.09 | 5.75 | 5.34 | 21.17 | * | 32.02 | 27.55 | N/A |
| 1-Kestose | 6.79 | 5.75 | 5.26 | 13.09 | * | － | 20.11 | N/A |
| Kojibiose | 7.56 | 6.21 | 5.88 | 9.65 | * | 11.47 | 14.82 | N/A |
| Lactitol | 13.27 | 8.09 | 6.13 | 16.35 | 6.67 | 14.04 | 11.82 | 13.28 |
| Lactose | 8.05 | 6.51 | 5.99 | 10.12 | 4.07 | 11.69 | 13.27 | 12.46 |
| Lactosyl fructoside | 7.12 | 5.88 | 5.29 | 14.69 | * | 15.75 | 18.98 | N/A |
| Lactulose | 9.13 | 6.99 | 6.19 | 9.16 | 4.65 | 10.80 | 10.72 | 10.52 |
| Maltitol | 12.23 | 8.26 | 6.03 | 13.04 | 6.77 | 11.81 | 11.82 | 12.37 |
| Maltose | 7.85 | 6.34 | 5.94 | 8.67 | * | 10.61 | 14.24 | 12.71 |
| Maltotriose | 7.48 | 5.89 | 5.38 | 13.79 | * | 17.88 | 24.96 | N/A |
| Mannitol | 15.80 | 11.10 | 7.23 | 8.75 | 9.03 | 6.84 | 7.39 | 7.35 |
| D-Mannose | 10.72 | 8.17 | 7.64 | 5.83 | 5.01 | 6.72 | 7.84 | 6.95 |
| D(+)-Melezitose | 6.94 | 5.79 | 5.24 | 13.60 | * | 14.68 | 19.27 | 18.67 |
| Melibiose | 8.16 | 6.45 | 5.98 | 11.69 | 4.23 | 14.83 | 14.70 | 15.07 |
| Methyl-α-D-mannopyranoside | 11.13 | 8.87 | 7.78 | 3.99 | 4.39 | 4.15 | 4.71 | N/A |
| Nystose | 6.38 | 5.45 | 4.93 | 20.05 | * | － | 31.90 | N/A |
| Palatinit | 2 peaks | 2 peaks | 5.90 | 2 peaks | 2 peaks | 2 peaks | 12.73 | 14.23 |
| Palatinose | 7.84 | 6.45 | 5.89 | 8.08 | 3.99 | 9.81 | 12.12 | 11.26 |
| Panose | 7.14 | 5.78 | 5.32 | 16.87 | * | 23.14 | 25.60 | N/A |
| L-Phenylalanine | － | － | － | － | 31.58 | － | － | N/A |
| D(+)-Raffinose | 7.14 | 5.78 | 5.29 | 16.36 | * | 19.11 | 20.25 | N/A |
| D(+)-Rhamnose | 9.77 | 8.23 | 7.37 | 3.93 | 4.43 | 4.09 | 5.52 | 4.87 |
| D(-)-Ribose | 19.35 | 13.66 | 9.04 | 4.82 | 8.64 | 5.30 | 5.45 | 4.93 |
| Rutinose | 7.81 | 6.49 | 5.80 | 6.65 | * | 7.21 | 10.87 | N/A |
| Saccharin sodium | 6.96 | 5.44 | 4.33 | 6.77 | * | 2.04 | 5.68 | N/A |
| D(-)-Sorbitol | 21.61 | 13.31 | 7.42 | 9.79 | 11.88 | 7.27 | 7.09 | 7.18 |
| D(+)-Sorbose | 9.67 | 8.03 | 7.38 | 5.12 | 4.92 | 5.91 | 7.35 | 6.79 |
| Stachyose | 6.82 | 5.57 | 4.97 | － | * | － | 36.22 | N/A |
| Stevioside | － | － | － | 4.14 | * | 4.44 | 6.07 | N/A |
| Sucrose | 7.54 | 6.29 | 5.87 | 7.91 | * | 8.68 | 11.87 | 10.69 |
| α-D-Talose | 21.33 | 12.59 | 8.76 | 5.69 | 8.51 | 6.32 | 6.47 | 5.75 |
| Theanderose | 2 peaks | 2 peaks | 2 peaks | 2 peaks | * | N/A | 2 peaks | N/A |
| Trehalose | 7.62 | 6.27 | 5.78 | 10.85 | * | 11.49 | 13.25 | 14.36 |
| Trehalulose | 8.92 | 6.95 | 6.10 | 9.54 | 4.78 | 11.38 | 11.68 | 11.92 |
| Xylitol | 19.87 | 13.14 | 7.94 | 7.77 | 10.16 | 6.19 | 6.10 | 6.04 |
| Xylobiose | 8.16 | 6.68 | 6.40 | 5.65 | * | 6.71 | 9.05 | 7.83 |
| D(+)-Xylose | 9.21 | 7.90 | 7.71 | 4.55 | 4.48 | 5.21 | 6.58 | 5.78 |
| D-Xylulose | 10.64 | 9.02 | 8.04 | 4.06 | 5.07 | 4.56 | 5.41 | N/A |
- N/A
- : Data not available
- (－)
- : Peak not detected
- (*)
- : Overlaps with solvent peak
- Column
- ：Shodex SUGAR SP0810, SC1011, KS-801 (8.0 mm I.D. x 300 mm each)
- Eluent
- ：H2O
- Flow rate
- ：1.0 mL/min
- Detector
- ：RI
- Column temp.
- ：80 ℃
- Column
- ：Shodex SUGAR SZ5532 (6.0 mm I.D. x 150 mm)
- Eluent
- ：H2O/CH3CN=25/75
- Flow rate
- ：1.0 mL/min
- Detector
- ：RI
- Column temp.
- ：60 ℃
- Column
- ：Shodex SUGAR SC1211 (6.0 mm I.D. x 250 mm)
- Eluent
- ：H2O/CH3CN=65/35
- Flow rate
- ：1.0 mL/min
- Detector
- ：RI
- Column temp.
- ：70 ℃
- Column
- ：Shodex RSpak DC-613 (6.0 mm I.D. x 150 mm)
- Eluent
- ：H2O/CH3CN=25/75
- Flow rate
- ：1.0 mL/min
- Detector
- ：RI
- Column temp.
- ：70 ℃
- Column
- ：Shodex Asahipak NH2P-50 4E (4.6 mm I.D. x 250 mm)
- Eluent
- ：H2O/CH3CN=25/75
- Flow rate
- ：1.0 mL/min
- Detector
- ：RI
- Column temp.
- ：30 ℃
- Column
- ：Shodex HILICpak VG-50 4E (4.6 mm I.D. x 250 mm)
- Eluent
- ：H2O/CH3CN=20/80
- Flow rate
- ：1.0 mL/min
- Detector
- ：RI
- Column temp.
- ：40 ℃
Sample Name Index
- 1-Fructofuranosyl-D-nystose
- 1-Kestose
- 2-Deoxy-D-glucose
- 4'-Galactosyllactose
- meso-Erythritol
- myo-Inositol
- Arabinose
- Arabitol
- Aspartame
- Difructose anhydride Ⅲ
- Ethanol, Ethyl alcohol
- Fructose
- Fucose
- Galactitol, Dulcitol
- Galactose
- Galacturonic acid
- Gentiobiose
- Glucose
- Glycerol, Glycerin
- Glycyrrhizin, Glycyrrhizic acid
- Isomaltose
- Isomaltotriose
- Kojibiose
- Lactitol
- Lactose
- Lactosyl fructoside
- Lactulose
- Maltitol
- Maltose
- Maltotriose
- Mannitol
- Mannose
- Melezitose
- Melibiose
- Methyl-α-D-mannopyranoside
- N-Acetyl-D-glucosamine
- Nystose
- Palatinit, Isomalt
- Palatinose, Isomaltulose
- Panose
- Phenylalanine, Phe
- Raffinose
- Rhamnose
- Ribose
- Rutinose
- Saccharin sodium
- Sorbitol, Glucitol
- Sorbose
- Stachyose
- Stevioside
- Sucrose, Saccharose
- Talose
- Theanderose
- Trehalose
- Trehalulose
- Xylitol
- Xylobiose
- Xylose
- Xylulose
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Elution Volume of Saccharides (VG-50 4E)
- Elution Volume of Saccharides (VG-25 4D)
- Separation of Anomer
- Comparison of Amino Column with Amide Column
- Comparison of Columns with Different Counter Ions
- Combination of Columns with Different Counter Ions
- Effects of Free Base Ratio of Amino Group on Plate Number (NH2P-50 4E)
- Effects of Acetonitrile Concentration on Elution Time (NH2P-50 4E)
- Effects of Acetonitrile Concentration on Elution Time (SZ5532)
- Effects of Acetonitrile Concentration on Plate Number (NH2P-50 4E)
- Durability Against Acidic Solvents (NH2P-50 4E)
- Durability Against Alkaline Solvents (NH2P-50 4E)
- Durability Against Eluent Composition Change (NH2P-50 4E)
- Effects of Sample Solvent Composition (NH2P-50 4E)
- Effects of Sample Injection Volume (NH2P-50 4E)
- Effects of Flow Rate (SC1011)
- Effects of Temperature (1) (SC1011)
- Effects of Temperature (2)
- Calibration Curves for Saccharides (NH2P-50 4E)
- Calibration Curves for Saccharides (SZ5532)
- Comparison of NH2P with Silica-based Amino Column (1)
- Comparison of NH2P with Silica-based Amino Column (2)
- Monosaccharides (1) (NH2P-50 4E)
- Monosaccharides (2) (NH2P-50 4E)
- Monosaccharides and Disaccharides (1) (SP0810)
- Monosaccharides and Disaccharides (2) (NH2P-50 4E)
- Monosaccharides and Disaccharides (3) (NH2P-50 4E)
- Monosaccharides and Disaccharides (4) (SZ5532)
- Monosaccharides and Disaccharides (5) (VG-50 4E)
- Mono-, Di- and Trisaccharides (1) (NH2P-50 4E)
- Saccharides in Agricultural Products (SC1011)
- Saccharides in Wood (1) (SP0810)
- Saccharides in Wood (2) (SP0810)
- Saccharides in Wood (3) (NH2P-50 4E)
- Palatinose in Food (SC1011)
- Worcester Sauce (KS-801)
- Yogurt: Sugar added (SP0810)
- Yogurt: Sugar added (NH2P-50 4E)
- Roast Sweet Potato (NH2P-50 4E)
- Roast Sweet Potato (SP0810)
- Soybean Flour (SP0810)
- Milk Coffee (NH2P-50 4E)
- Jelly (NH2P-50 4E)
- Saccharides in Bread (KS-801)
- Lactic acid Beverage and Fermented Milk (SH1011)
- Orange and Melon (SH1011)
- Orange Juice (SC1011)
- Chocolate Cake (NH2P-50 4E)
- Chocolate Cake (SZ5532)
- Maltose and Isomaltose (SZ5532)
- Saccharide Analysis Using Semi-micro Column (NH2P-50 2D)
- High Sensitive Analysis of Saccharides in powdered Milk (NH2P-50 4E)
- Glucose Derivatives (NH2P-50 4E)
- Extract of Wheat Rod (NH2P-50 4E)
- Effects of Organic Solvent in Eluent (NH2P-50 4E)
- Saccharides in Food (SC1011)
- Monosaccharides Composing Sugar Chain (SH1011)
- Maltose and Nigerose (NH2P-50 4E)
- Analysis of Ketohexose (SP0810)
- Cello-oligosaccharides and Ethanol (KS-802)
- Analysis of Rare Sugar (1) (SP0810)
- Analysis of Rare Sugar (2) (VG-50 4E)
- Galactinol (SC1011)
- Saccharides Separation in Presence of Na Salt (DC-613)
- Heptulose Separation (KS-801)
- Saccharides Analysis using Corona Charged Aerosol Detector (NH2P-50 4E)
- Comparison between NH2P-40 3E and its Conventional Type (NH2P-50 4E)
- Analysis of Saccharides and Furfurals (KS-801)
- Saccharides Analysis in Ionic Liquid (DC-613)
- Gulose (SP0810)
- Simultaneous Analysis of Lactose, Epilactose, and Lactulose (VG-50 4E)
- Analysis of Aldoses (VG-50 4E)
- Abnormal Behavior of Chromatogram of Saccharides Using a Pb Type Column for Ligand Exchange Chromatography
- Analysis of Fructose Glucose Syrup (High Fructose Syrup) According to Japanese Pharmaceutical Excipients Method (KS-801)
- Analysis of Maltose Starch Powder According to Japanese Pharmaceutical Excipients Method (KS-801)
- Analysis of Ribose According to USP-NF Method (KS-801)
- Analysis of Dextrose According to USP-NF Method (SC1011)
- Analysis of Tagatose According to USP-NF Method (SC1011)
- Analysis of Maltose According to USP-NF Method (KS-801)
- Analysis of Trehalose According to USP-NF Method (KS-801)
- Analysis of Maltose Hydrate According to JP Method (KS-801)
- Analysis of Trehalose According to JP Method (KS-801)
- Analysis of Lactulose According to JP Method (SP0810)
- Analysis of Ethyl α-D-Glucoside in Sake (VG-50 4E)
- Analysis of Erythrose and Threose (SC1011)
- Analysis of 2-Deoxy-D-glucose (SC1011)
- Analysis of 2-Deoxy-D-glucose (VG-50 4E)
- Analysis of Dextrose and Fructose in Cranberry Juice (NH2P-50 4E)