Elution Volume of Saccharides (VG-50 4E)

Below table summarizes elution volumes of saccharides using HILICpak VG-50 4E, a polymer-based amino column. Eluent 4 condition is recommended for monosaccharides and sugar alcohols analyses. Note that disaccharides are retained strongly under the Eluent 4 condition, so not recommended for the samples containing disaccharides. Elution values shown are the peak top positions, please contact us to request a chromatogram if you would like to see their elution patterns. 

Click here for a list of “Elution Volume of Saccharides” using other sugar analysis columns.
Click here for a list of “Elution Volume of Saccharides” using HILICpak VG-25 4D.

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Substance Elution Volume (mL)
Eluent 1 Eluent 2 Eluent 3 Eluent 4
Arabinose   7.36   6.54   5.65 12.80
Arabitol   8.17   7.14   6.05   9.08
meso-Erythritol   6.22   5.60   5.10   6.65
Fructose   8.66   7.53   6.19 12.57
Fucose   6.33   5.71   5.06   9.36
Galactose 11.16   9.42   7.37 19.20
Gentiobiose 36.57 27.17 14.52 N/A
Glucose 11.97 10.15   7.47 21.95
myo-Inositol 21.43 16.87 11.68 N/A
Isomaltose 37.29 28.23 14.41 N/A
Lactitol 28.87 21.48 13.28 N/A
Lactose 26.94 20.31 12.46 N/A
Lactulose 20.90 15.93 10.52 N/A
Maltitol 27.79 21.44 12.37 N/A
Maltose 29.93 23.38 12.71 N/A
Mannitol 11.27   9.61   7.35 12.63
Mannose 10.73   9.26   6.95 20.49
Melezitose 35.84 46.47 18.67 N/A
Melibiose 39.07 26.92 15.07 N/A
Palatinit 2 peaks 2 peaks 14.23 N/A
Palatinose 24.40 19.00 11.26 N/A
Rhamnose   6.00   5.49   4.87   9.33
Ribose   6.04   5.54   4.93 10.83
Sorbitol 10.48   8.92   7.18 12.11
Sorbose   9.28   8.08   6.79 14.55
Sucrose 22.16 17.46 10.69 N/A
Talose   7.90   7.03   5.75 15.71
Trehalose 37.98 29.74 14.36 N/A
Trehalulose 26.39 20.49 11.92 N/A
Xylitol   7.88   6.89   6.04   8.90
Xylobiose 12.59 10.31   7.83 N/A
Xylose   7.72   6.89   5.78 14.16
N/A
: Data not available
Column
Shodex HILICpak VG-50 4E (4.6 mm I.D. x 250 mm)
Eluent
(1) H2O/CH3CN=12/88
(2) H2O/CH3OH/CH3CN=10/5/85
(3) H2O/CH3CN=20/80
(4) (8 mM NaOH + 5 mM NaCl aq.)/CH3CN=18/82
Flow rate
1.0 mL/min
Detector
RI
Column temp.
(Eluent 1 and 2) 60 ℃
(Eluent 3 and 4) 40 ℃

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