Below table summarizes elution volumes of saccharides using HILICpak VG-50 4E, a polymer-based amino column. Eluent 4 condition is recommended for monosaccharides and sugar alcohols analyses. Note that disaccharides are retained strongly under the Eluent 4 condition, so not recommended for the samples containing disaccharides. Elution values shown are the peak top positions, please contact us to request a chromatogram if you would like to see their elution patterns.
Click here for a list of “Elution Volume of Saccharides” using other sugar analysis columns.
Click here for a list of “Elution Volume of Saccharides” using HILICpak VG-25 4D.
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| Substance | Elution Volume (mL) | |||
|---|---|---|---|---|
| Eluent 1 | Eluent 2 | Eluent 3 | Eluent 4 | |
| Arabinose | 7.36 | 6.54 | 5.65 | 12.80 |
| Arabitol | 8.17 | 7.14 | 6.05 | 9.08 |
| meso-Erythritol | 6.22 | 5.60 | 5.10 | 6.65 |
| Fructose | 8.66 | 7.53 | 6.19 | 12.57 |
| Fucose | 6.33 | 5.71 | 5.06 | 9.36 |
| Galactose | 11.16 | 9.42 | 7.37 | 19.20 |
| Gentiobiose | 36.57 | 27.17 | 14.52 | N/A |
| Glucose | 11.97 | 10.15 | 7.47 | 21.95 |
| myo-Inositol | 21.43 | 16.87 | 11.68 | N/A |
| Isomaltose | 37.29 | 28.23 | 14.41 | N/A |
| Lactitol | 28.87 | 21.48 | 13.28 | N/A |
| Lactose | 26.94 | 20.31 | 12.46 | N/A |
| Lactulose | 20.90 | 15.93 | 10.52 | N/A |
| Maltitol | 27.79 | 21.44 | 12.37 | N/A |
| Maltose | 29.93 | 23.38 | 12.71 | N/A |
| Mannitol | 11.27 | 9.61 | 7.35 | 12.63 |
| Mannose | 10.73 | 9.26 | 6.95 | 20.49 |
| Melezitose | 35.84 | 46.47 | 18.67 | N/A |
| Melibiose | 39.07 | 26.92 | 15.07 | N/A |
| Palatinit | 2 peaks | 2 peaks | 14.23 | N/A |
| Palatinose | 24.40 | 19.00 | 11.26 | N/A |
| Rhamnose | 6.00 | 5.49 | 4.87 | 9.33 |
| Ribose | 6.04 | 5.54 | 4.93 | 10.83 |
| Sorbitol | 10.48 | 8.92 | 7.18 | 12.11 |
| Sorbose | 9.28 | 8.08 | 6.79 | 14.55 |
| Sucrose | 22.16 | 17.46 | 10.69 | N/A |
| Talose | 7.90 | 7.03 | 5.75 | 15.71 |
| Trehalose | 37.98 | 29.74 | 14.36 | N/A |
| Trehalulose | 26.39 | 20.49 | 11.92 | N/A |
| Xylitol | 7.88 | 6.89 | 6.04 | 8.90 |
| Xylobiose | 12.59 | 10.31 | 7.83 | N/A |
| Xylose | 7.72 | 6.89 | 5.78 | 14.16 |
- N/A
- : Data not available
- Column
- ：Shodex HILICpak VG-50 4E (4.6 mm I.D. x 250 mm)
- Eluent
- ：(1) H2O/CH3CN=12/88
(2) H2O/CH3OH/CH3CN=10/5/85
(3) H2O/CH3CN=20/80
(4) (8 mM NaOH + 5 mM NaCl aq.)/CH3CN=18/82 - Flow rate
- ：1.0 mL/min
- Detector
- ：RI
- Column temp.
- ：(Eluent 1 and 2) 60 ℃
(Eluent 3 and 4) 40 ℃
Sample Name Index
- meso-Erythritol
- myo-Inositol
- Arabinose
- Arabitol
- Fructose
- Fucose
- Galactose
- Gentiobiose
- Glucose
- Isomaltose
- Isomaltotriose
- Lactitol
- Lactose
- Lactulose
- Maltitol
- Maltose
- Maltotriose
- Mannitol
- Mannose
- Melezitose
- Melibiose
- Palatinit, Isomalt
- Palatinose, Isomaltulose
- Rhamnose
- Ribose
- Sorbitol, Glucitol
- Sorbose
- Sucrose, Saccharose
- Talose
- Trehalose
- Trehalulose
- Xylitol
- Xylobiose
- Xylose
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Elution Volume of Saccharides
- Elution Volume of Saccharides (VG-25 4D)
- Separation of Anomer
- Comparison of Amino Column with Amide Column
- Comparison of Columns with Different Counter Ions
- Combination of Columns with Different Counter Ions
- Effects of Free Base Ratio of Amino Group on Plate Number (NH2P-50 4E)
- Effects of Acetonitrile Concentration on Elution Time (NH2P-50 4E)
- Effects of Acetonitrile Concentration on Elution Time (SZ5532)
- Effects of Acetonitrile Concentration on Plate Number (NH2P-50 4E)
- Durability Against Acidic Solvents (NH2P-50 4E)
- Durability Against Alkaline Solvents (NH2P-50 4E)
- Durability Against Eluent Composition Change (NH2P-50 4E)
- Effects of Sample Solvent Composition (NH2P-50 4E)
- Effects of Sample Injection Volume (NH2P-50 4E)
- Effects of Flow Rate (SC1011)
- Effects of Temperature (1) (SC1011)
- Effects of Temperature (2)
- Calibration Curves for Saccharides (NH2P-50 4E)
- Calibration Curves for Saccharides (SZ5532)
- Comparison of NH2P with Silica-based Amino Column (1)
- Comparison of NH2P with Silica-based Amino Column (2)
- Monosaccharides (1) (NH2P-50 4E)
- Monosaccharides (2) (NH2P-50 4E)
- Monosaccharides and Disaccharides (1) (SP0810)
- Monosaccharides and Disaccharides (2) (NH2P-50 4E)
- Monosaccharides and Disaccharides (3) (NH2P-50 4E)
- Monosaccharides and Disaccharides (4) (SZ5532)
- Monosaccharides and Disaccharides (5) (VG-50 4E)
- Monosaccharides and Disaccharides (8) (VG-25 4D)
- Mono-, Di- and Trisaccharides (1) (NH2P-50 4E)
- Saccharides in Agricultural Products (SC1011)
- Saccharides in Wood (1) (SP0810)
- Saccharides in Wood (2) (SP0810)
- Saccharides in Wood (3) (NH2P-50 4E)
- Palatinose in Food (SC1011)
- Worcester Sauce (KS-801)
- Yogurt: Sugar added (SP0810)
- Yogurt: Sugar added (NH2P-50 4E)
- Roast Sweet Potato (NH2P-50 4E)
- Roast Sweet Potato (SP0810)
- Soybean Flour (SP0810)
- Milk Coffee (NH2P-50 4E)
- Jelly (NH2P-50 4E)
- Saccharides in Bread (KS-801)
- Lactic acid Beverage and Fermented Milk (SH1011)
- Orange and Melon (SH1011)
- Orange Juice (SC1011)
- Chocolate Cake (NH2P-50 4E)
- Chocolate Cake (SZ5532)
- Maltose and Isomaltose (SZ5532)
- Saccharide Analysis Using Semi-micro Column (NH2P-50 2D)
- High Sensitive Analysis of Saccharides in powdered Milk (NH2P-50 4E)
- Glucose Derivatives (NH2P-50 4E)
- Extract of Wheat Rod (NH2P-50 4E)
- Effects of Organic Solvent in Eluent (NH2P-50 4E)
- Saccharides in Food (SC1011)
- Monosaccharides Composing Sugar Chain (SH1011)
- Maltose and Nigerose (NH2P-50 4E)
- Analysis of Ketohexose (SP0810)
- Cello-oligosaccharides and Ethanol (KS-802)
- Analysis of Rare Sugar (1) (SP0810)
- Analysis of Rare Sugar (2) (VG-50 4E)
- Galactinol (SC1011)
- Saccharides Separation in Presence of Na Salt (DC-613)
- Heptulose Separation (KS-801)
- Saccharides Analysis using Corona Charged Aerosol Detector (NH2P-50 4E)
- Comparison between NH2P-40 3E and its Conventional Type (NH2P-50 4E)
- Analysis of Saccharides and Furfurals (KS-801)
- Saccharides Analysis in Ionic Liquid (DC-613)
- Gulose (SP0810)
- Simultaneous Analysis of Lactose, Epilactose, and Lactulose (VG-50 4E)
- Simultaneous Analysis of Lactose, Epilactose, and Lactulose (2) (VG-25 4D)
- Analysis of Aldoses (VG-50 4E)
- Abnormal Behavior of Chromatogram of Saccharides Using a Pb Type Column for Ligand Exchange Chromatography
- Analysis of Fructose Glucose Syrup (High Fructose Syrup) According to Japanese Pharmaceutical Excipients Method (KS-801)
- Analysis of Maltose Starch Powder According to Japanese Pharmaceutical Excipients Method (KS-801)
- Analysis of Ribose According to USP-NF Method (KS-801)
- Analysis of Dextrose According to USP-NF Method (SC1011)
- Analysis of Tagatose According to USP-NF Method (SC1011)
- Analysis of Maltose According to USP-NF Method (KS-801)
- Analysis of Trehalose According to USP-NF Method (KS-801)
- Analysis of Maltose Hydrate According to JP Method (KS-801)
- Analysis of Trehalose According to JP Method (KS-801)
- Analysis of Lactulose According to JP Method (SP0810)
- Analysis of L-Rhamnose in Accordance with the Japan’s Specifications and Standards for Food Additives (NH2P-50 4D)
- Analysis of Ethyl α-D-Glucoside in Sake (VG-50 4E)
- Analysis of Erythrose and Threose (SC1011)
- Analysis of 2-Deoxy-D-glucose (SC1011)
- Analysis of 2-Deoxy-D-glucose (VG-50 4E)
- Analysis of Dextrose and Fructose in Cranberry Juice (NH2P-50 4E)
- Simultaneous Analysis of 15 Components Including Monosaccharides, Disaccharides, and Oligosaccharides by HILIC Mode (VG-50 4E)