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Effects of Organic Solvent in Eluent

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Effects of organic solvent in eluent when saccharides are analyzed using Asahipak NH2P-50 4E (a column for saccharides analysis) is shown.


Sample :
1. Fructose
2. Glucose
3. Sucrose
4. Lactose
5. Maltose


Column       : Shodex Asahipak NH2P-50 4E (4.6mmI.D. x 250mm)
Eluent       : (left); H2O/CH3CN=25/75
               (right); H2O/C2H5OH=10/90 
Flow rate    : 0.6mL/min
Detector     : Shodex RI
Column temp. : 30°C

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