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Orange Juice


Saccharides in orange juice were analyzed using SUGAR SC1011 (a column for saccharides analysis). For the pretreatment of juice, first, the sample was filtrated 2 times using a 0.45μm disposal filter. Then, it was treated with a mini-column packed with anion ion exchanger. And it was filtered again. In front of glucose and fructose peaks, a large peak was observed and it was considered to be oligosaccharides.

Sample : Mandarin orange juice, 5μL
1. Sucrose
2. Glucose
3. Fructose

Column       : Shodex SUGAR SC1011 (8.0mmI.D. x 300mm)
Eluent       : H2O
Flow rate    : 1.0mL/min
Detector     : Shodex RI
Column temp. : 80°C

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