Saccharides in orange juice were analyzed using SUGAR SC1011
(a column for saccharides analysis). For the pretreatment of juice, first, the
sample was filtrated 2 times using a 0.45μm disposal filter. Then, it was
treated with a mini-column packed with anion ion exchanger. And it was filtered
again. In front of glucose and fructose peaks, a large peak was observed and it
was considered to be oligosaccharides.
Sample : Mandarin orange
juice, 5μL
1. Sucrose
2. Glucose
3. Fructose
Column : Shodex SUGAR SC1011 (8.0mmI.D. x 300mm) Eluent : H2O Flow rate : 1.0mL/min Detector : Shodex RI Column temp. : 80°C
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