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Comparison of NH2P with Silica-based Amino Column (1)


Polymer-based Asahipak NH2P-50 4E column has resolved the problem occurring with silica-based amino columns, whose performance falls with the time.
1) Conventional analytical methods used for silica-based amino columns can be applied to the NH2P column.
Eluent ---- Mixture of water and acetonitrile which is commonly used for silica-based amino columns can be also used.
Column temperature ---- Can be analyzed at room temperature
2) Excellent quantitative analysis can be performed using NH2P column.
3) Further more, the NH2P column can be washed with alkaline solvent.

Sample :
1. Fructose
2. Glucose
3. Sucrose
4. Maltose

Columns      : Shodex Asahipak NH2P-50 4E, Amino column from other manufacturer
               (4.6mmI.D. x 250mm each)
Eluent       : CH3CN/H2O=75/25
Flow rate    : 1.0mL/min
Detector     : Shodex RI
Column temp. : 30°C

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