Two sugar analysis columns, SUGAR SP0810, was used to analyze saccharides in wood. Corona charged aerosol detector (CAD) was used as a detector. Comparing to Sacchrides in Wood (1) , the separation of arabinose and mannose was improved by adding CH3CN to the mobile phase.
Sample : 10 μL
1. Cellobiose 1.0 %
2. Glucose 1.5 %
3. Xylose 0.5 %
4. Galactose 0.5 %
5. Arabinose 0.5 %
6. Mannose 1.0 %
Column : Shodex SUGAR SP0810 (8.0 mm I.D. x 300 mm) x 2 Eluent : H2O/CH3CN=87/13 Flow rate : 0.6 mL/min Detector : Corona charged aerosol Column temp. : 85 °C
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