Analysis of Maltose Starch Powder According to Japanese Pharmaceutical Excipients Method (KS-801)

According to Japanese Pharmaceutical Excipients 2013*, “Assay" for “Maltose Syrup Powder" should be carried out with a column filled with strong acidic ion-exchange resin consist of styrene divinylbenzene copolymer carrying sulfonic acid groups. The SUGAR KS-801 confirmed the requirements were met.

Selection of columnElution order (Solution for selection of column): maltotriose, maltose, glucose
Resolution between maltotriose and maltose (Solution for selection of column): ≥ 1.6
Resolution between maltose and glucose (Solution for selection of column): ≥ 4.1

*The version at the time of the application acquisition.

Sample: 10 µL
(Solution for selection of column) 5 mg/mL each of Maltotriose, Maltose and Glucose in H2O

  1. 1.Maltotriose
  2. 2.Maltose
  3. 3.Glucose
Column
Shodex SUGAR KS-801 (8.0 mm I.D. x 300 mm)
Eluent
H2O
Flow rate
0.4 mL/min
Detector
RI
Column temp.
50 ℃

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