Durability Against Acidic Solvents (NH2P-50 4E)

Since Asahipak NH2P-50 column is chemically stable, the durability against acidic solvents is better than that of silica-based amino columns.

Testing procedureScroll
(1) Testing (63 hrs)
Eluent0.01 M H2SO4 (pH2)
Flow rate0.1 mL/min
Column temp.Room temp.
(2) Replacement (1 hr)
Flow rate0.5 mL/min
Column temp.Room temp.
(3) Equilibrium (1 hr)
Eluent0.1 M NaOH aq.
Flow rate0.5 mL/min
Column temp.Room temp.
Comparison between before and after testingScroll
No. Saccharides Before testing After testing
Elution volume (mL) Plate number Elution volume (mL) Plate number
1 Fructose   6.73 9600   6.70 8400
2 Glucose   8.59 7900   8.58 8500
3 Sucrose 11.88 9600 11.93 9300
4 Maltose 14.24 8500 13.90 8600
Shodex Asahipak NH2P-50 4E (4.6 mm I.D. x 250 mm)
Flow rate
:1.0  mL/min
Column temp.
30 ℃

Sample Name Index

Operation Manual / Certificate of Analysis

Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.

Product Name Index


Applications (Related Information)