Since Asahipak NH2P-50 column is chemically stable, the durability against acidic solvents is better than that of silica-based amino columns.
Testing procedure
(1)Testing(63 hrs) Eluent : 0.01 M H2SO4 (pH2) Flow rate : 0.1 mL/min Column temp. : Room temp. |
(2)Replacement(1 hr) Eluent : H2O Flow rate : 0.5 mL/min Column temp. : Room temp. |
(3)Equilibrium(1 hr) Eluent : 0.1 M NaOH Flow rate : 0.5 mL/min Column temp. : Room temp. |
Comparison between before and after testing | ||||
---|---|---|---|---|
Sample | Before testing | After testing | ||
Elution volume (mL) | Plate number | Elution volume (mL) | Plate number | |
1.Fructose | 6.73 | 9600 | 6.70 | 8400 |
2.Glucose | 8.59 | 7900 | 8.58 | 8500 |
3.Sucrose | 11.88 | 9600 | 11.93 | 9300 |
4.Maltose | 14.24 | 8500 | 13.90 | 8600 |
Column : Shodex Asahipak NH2P-50 4E (4.6 mm I.D. x 250 mm) Eluent : CH3CN/H2O=75/25 Flow rate : 1.0 mL/min Detector : RI Column temp. : 30 °C
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