Shodex™ Capture the Essence
Durability Against Acidic Solvents (NH2P-50 4E)
Since Asahipak NH2P-50 column is chemically stable, the durability against acidic solvents is better than that of silica-based amino columns.
Testing procedure
| (1)Testing(63 hrs) Eluent : 0.01 M H2SO4 (pH2) Flow rate : 0.1 mL/min Column temp. : Room temp. |
(2)Replacement(1 hr) Eluent : H2O Flow rate : 0.5 mL/min Column temp. : Room temp. |
(3)Equilibrium(1 hr) Eluent : 0.1 M NaOH Flow rate : 0.5 mL/min Column temp. : Room temp. |
| Comparison between before and after testing | ||||
|---|---|---|---|---|
| Sample | Before testing | After testing | ||
| Elution volume (mL) | Plate number | Elution volume (mL) | Plate number | |
| 1.Fructose | 6.73 | 9600 | 6.70 | 8400 |
| 2.Glucose | 8.59 | 7900 | 8.58 | 8500 |
| 3.Sucrose | 11.88 | 9600 | 11.93 | 9300 |
| 4.Maltose | 14.24 | 8500 | 13.90 | 8600 |
Column : Shodex Asahipak NH2P-50 4E (4.6 mm I.D. x 250 mm) Eluent : CH3CN/H2O=75/25 Flow rate : 1.0 mL/min Detector : RI Column temp. : 30 °C
Sample name index
Product name index
Applications
- Elution Volume of Saccharides
- Elution Volume of Saccharides (VG-50 4E)
- Separation of Anomer
- Comparison of Amino Column with Amide Column
- Comparison of Columns with Different Counter Ions
- Combination of Columns with Different Counter Ions
- Effects of Free Base Ratio of Amino Group on Plate Number
- Effects of Acetonitrile Concentration on Elution Time (NH2P-50 4E)
- Effects of Acetonitrile Concentration on Elution Time (SZ5532)
- Effects of Acetonitrile Concentration on Plate Number
- Durability Against Alkaline Solvents (NH2P-50 4E)
- Durability Against Eluent Composition Change (NH2P-50 4E)
- Effects of Sample Solvent Composition (NH2P-50 4E)
- Effects of Sample Injection Volume (NH2P-50 4E)
- Effects of Flow Rate (SC1011)
- Effects of Temperature (1) (SC1011)
- Effects of Temperature (2)
- Calibration Curves for Saccharides (NH2P-50 4E)
- Calibration Curves for Saccharides (SZ5532)
- Comparison of NH2P with Silica-based Amino Column (1)
- Comparison of NH2P with Silica-based Amino Column (2)
- Monosaccharides (1) (NH2P-50 4E)
- Monosaccharides (2) (NH2P-50 4E)
- Monosaccharides and Disaccharides (1) (SP0810)
- Monosaccharides and Disaccharides (2) (NH2P-50 4E)
- Monosaccharides and Disaccharides (3) (NH2P-50 4E)
- Mono-, Di- and Trisaccharides (1) (NH2P-50 4E)
- Saccharides in Agricultural Products (SC1011)
- Saccharides in Wood (1) (SP0810)
- Saccharides in Wood (2) (SP0810)
- Palatinose in Food (SC1011)
- Worcester Sauce (KS-801)
- Yogurt: Sugar added (SP0810)
- Yogurt: Sugar added (NH2P-50 4E)
- Roast Sweet Potato (NH2P-50 4E)
- Roast Sweet Potato (SP0810)
- Soybean Flour (SP0810)
- Milk Coffee (NH2P-50 4E)
- Jelly (NH2P-50 4E)
- Saccharides in Bread (KS-801)
- Candy (SC1011 and SC1821)
- Lactic acid Beverage and Fermented Milk (SH1011)
- Orange and Melon (SH1011)
- Orange Juice (SC1011)
- Chocolate Cake (NH2P-50 4E)
- Chocolate Cake (SZ5532)
- Maltose and Isomaltose (SZ5532)
- Saccharide Analysis Using Semi-micro Column (NH2P-50 2D)
- High Sensitive Analysis of Saccharides in powdered Milk (NH2P-50 4E)
- Glucose Derivatives (NH2P-50 4E)
- Extract of Wheat Rod (NH2P-50 4E)
- Effects of Organic Solvent in Eluent (NH2P-50 4E)
- Saccharides in Food (SC1011)
- Monosaccharides Composing Sugar Chain (SH1011)
- Maltose and Nigerose (NH2P-50 4E)
- Analysis of Ketohexose (SP0810)
- Monosaccharides and Disaccharides (4) (SZ5532)
- Cello-oligosaccharides and Ethanol (KS-802)
- Analysis of Rare Sugar (1) (SP0810)
- Galactinol (SC1011)
- Analysis of Rare Sugar (2) (VG-50 4E)
- Saccharides Separation in Presence of Na Salt (DC-613)
- Heptulose Separation (KS-801)
- Saccharides Analysis using Corona Charged Aerosol Detector (NH2P-50 4E)
- Comparison between NH2P-40 3E and its Conventional Type (NH2P-50 4E)
- Analysis of Saccharides and Furfurals (KS-801)
- Saccharides Analysis in Ionic Liquid (DC-613)
- Gulose (SP0810)
- Analysis of Trehalose According to JP Method (KS-801)
- Analysis of Trehalose According to USP-NF Method (KS-801)
- Simultaneous Analysis of Lactose, Epilactose, and Lactulose (VG-50 4E)
- Saccharides in Wood (3) (NH2P-50 4E)
- Monosaccharides and Disaccharides (5) (VG-50 4E)
- Analysis of Aldoses (VG-50 4E)
- Abnormal Behavior of Chromatogram of Saccharides Using a Pb Type Column for Ligand Exchange Chromatography
- Analysis of Fructose Glucose Syrup According to Japanese Pharmaceutical Excipients Method (KS-801)
- Analysis of Maltose Starch Powder According to Japanese Pharmaceutical Excipients Method (KS-801)
- Analysis of Ribose According to USP-NF Method (KS-801)
- Analysis of Dextrose According to USP-NF Method (SC1011)
- Analysis of Tagatose According to USP-NF Method (SC1011)
- Analysis of Ethyl α-D-Glucoside in Sake (VG-50 4E)
- Analysis of Maltose According to USP-NF Method (KS-801)
- Analysis of Maltose Hydrate According to JP Method (KS-801)
- Analysis of Lactulose According to JP Method (SP0810)
- Analysis of Erythrose and Threose (SC1011)
- Analysis of 2-Deoxy-D-glucose (SC1011)
- Analysis of 2-Deoxy-D-glucose (VG-50 4E)
Applications (related information)
Saccharides and Organic Acids
- Monosaccharides and Oligosaccharides
- Oligosaccharides
- Acid Saccharides, Phosphorylated Saccharides
- Saccharides, Sugar Alcohols, Amino Sugars and Amino Acids
- Saccharides and Organic Acids
- Polysaccharides
- High-selective Analysis of Saccharides
- Sweeteners
- Organic Acids
- Guidelines for Shodex Column Selection : by Separation Mode
- Guidelines for Shodex Column Selection : by Sample Category
- Guidelines for Shodex Column Selection : by Elution Volume
- Guidelines for Shodex Column Selection : by Kinds of Saccharides
- Guidelines for Shodex Column Selection : by Combination of Saccharides
- Explanation on Ligand Exchange Mode
- Guidelines for Shodex Column Selection : Organic Acids
Shodex GLOBAL Website