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Durability Against Acidic Solvents

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Since Asahipak NH2P-50 column is chemically stable, the durability against acidic solvents is better than that of silica-based amino columns.

Testing procedure

(1)Testing(63hrs)
Eluent : 0.01M H2SO4(pH2)
Flow rate : 0.1mL/min
Column temp. : Room temp.
(2)Replacement(1hr)
Eluent : H2O
Flow rate : 0.5mL/min
Column temp. : Room temp.
(3)Equilibrium(1hr)
Eluent : 0.1M NaOH
Flow rate : 0.5mL/min
Column temp. : Room temp.


Comparison between before and after testing
SampleBefore testingAfter testing
Elution volume
(mL)
Plate numberElution volume
(mL)
Plate number
1.Fructose 6.73 9600 6.70 8400
2.Glucose 8.59 7900 8.58 8500
3.Sucrose 11.88 9600 11.93 9300
4.Maltose 14.24 8500 13.90 8600
Column       : Shodex Asahipak NH2P-50 4E (4.6mmI.D. x 250mm)
Eluent       : CH3CN/H2O=75/25
Flow rate    : 1.0mL/min
Detector     : Shodex RI
Column temp. : 30°C

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