Saccharides in chocolate cake were analyzed using SUGAR SZ5532 (a column for saccharides analysis).

(Sample pretreatment)
1) Take 5 g of chocolate cake in a vial for centrifuge, add 40 mL of petroleum ether and stir it for 15 minutes. Then use centrifuge for 10 minutes at 2,000 rpm and take off the supernatant.
2) Again, add 40 mL of petroleum ether and stir it for 15 minutes and take off remaining petroleum ether completely in a water bath of 40 °C.
3) Add 30 mL of pure water, apply ultrasonic vbvration for 30 minutes and make 50 mL of solution with pure water.
4) Filtrate with No.5B paper filter and ultrafiltrate at Mw 10,000.
5) Take a part of filtrated sample and add same quantity of acetonitrile.
6) Filtrate with 0.45 μm membrane filter.

Sample : Chocolate cake, 10 μL
1. Fructose
2. Glucose
3. Sucrose
4. Maltose
5. Lactose

Column       : Shodex SUGAR SZ5532 (6.0 mm I.D. x 150 mm)
Eluent       : CH3CN/H2O=75/25
Flow rate    : 0.6 mL/min
Detector     : RI
Column temp. : 60 °C

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