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Yogurt: Sugar added (SP0810)


Sugar added yogurt was analyzed using SUGAR SP0810.

(Sample pretreatment)
1) Take 5g of sugar added yogurt in a beaker, add 30mL of pure water and neutralize with 10%(w/v) NaOH.
2) Apply ultrasonic vibration for 30 minutes and make 50mL solution.
3) Filtrate with No.5B paper filter and then with 0.45μm membrane filter.

Sample : Yogurt, 5μL
1. Sucrose
2. Lactose
3. Glucose
4. Galactose

Column       : Shodex SUGAR SP0810 (8.0mmI.D. x 300mm)
Eluent       : H2O
Flow rate    : 0.6mL/min
Detector     : Shodex RI
Column temp. : 85°C

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