High Sensitive Analysis of Saccharides in powdered Milk (NH2P-50 4E)

High sensivie analysis of saccharides in powdered milk was performed using Asahipak NH2P-50 4E (a column for saccharides analysis) and an electrochmical detection system.


Sample : powdered Milk
1. Lactulose
2. Lactose


Instrument        : Agilent 1050 system
Reagent           : 0. 6 M LiOH 
Flow rate         : 0.8 mL/min
Mode              : pulse
Working electrode : Gold
Potential         : Pot 1=0.65 V, Pot 2=-0.95 V, Pot=0.15 V
Column : Shodex Asahipak NH2P-50 4E (4.6 mm I.D. x 250 mm) Eluent : (A); CH3CN/H2O=80/20 (B); H2O 0 min to 40 min, 5 % (B) to 40 % (B) 40 min to 45 min, 40 % (B) Flow rate : 1.0 mL/min Detector : Agilent 1049A Electrochemical Column temp. : 40 °C

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