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Durability Against Alkaline Solvents

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Since the packing material of Asahipak NH2P-50 column is polymer, it is durable against alkaline solvents. To the contrary, silica-based amino columns are not durable against alkaline solvents.

Testing procedure

(1)Equilibrium(1hr)
Conditions before testing
Eluent : 100mM Ammonium acetate(pH9.3)
Flow rate : 0.1mL/min
Column temp. : Room temp.
(2)Analysis


(3)Testing(160hrs)
Eluent : 5mM NaOH(pH11.4)/CH3CN=25/75
Flow rate : 0.1mL/min
Column temp. : 30°C
(4)Replacement(0.5hr)
Eluent : H2O
Flow rate : 0.5mL/min
Column temp. : Room temp.
(5)Replacement(0.5hr)
Eluent : H2O
Flow rate : 0.5mL/min
Column temp. : Room temp.
(6)Analysis




Comparison between before and after testing
SampleBefore testingAfter testing
Elution volume
(mL)
Plate numberElution volume
(mL)
Plate number
1.Fructose 7.52 7100 7.62 8100
2.Glucose 9.63 6100 9.68 7700
3.Sucrose 13.41 8400 13.40 9700
4.Maltose 16.16 7200 16.08 7400

Column       : Shodex Asahipak NH2P-50 4E (4.6mmI.D. x 250mm)
Eluent       : CH3CN/H2O=75/25
Flow rate    : 1.0mL/min
Detector     : Shodex RI
Column temp. : 30°C

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