Since the packing material of Asahipak NH2P-50 column is polymer, it is durable against alkaline solvents. To the contrary, silica-based amino columns are not durable against alkaline solvents.
Testing procedure
(1)Equilibrium(1hr) Conditions before testing Eluent : 100mM Ammonium acetate(pH9.3) Flow rate : 0.1mL/min Column temp. : Room temp. |
(2)Analysis |
(3)Testing(160hrs) Eluent : 5mM NaOH(pH11.4)/CH3CN=25/75 Flow rate : 0.1mL/min Column temp. : 30°C |
(4)Replacement(0.5hr)
Eluent : H2O Flow rate : 0.5mL/min Column temp. : Room temp. |
(5)Replacement(0.5hr)
Eluent : H2O Flow rate : 0.5mL/min Column temp. : Room temp. |
(6)Analysis |
Comparison between before and after testing | ||||
---|---|---|---|---|
Sample | Before testing | After testing | ||
Elution volume (mL) | Plate number | Elution volume (mL) | Plate number | |
1.Fructose | 7.52 | 7100 | 7.62 | 8100 |
2.Glucose | 9.63 | 6100 | 9.68 | 7700 |
3.Sucrose | 13.41 | 8400 | 13.40 | 9700 |
4.Maltose | 16.16 | 7200 | 16.08 | 7400 |
Column : Shodex Asahipak NH2P-50 4E (4.6mmI.D. x 250mm) Eluent : CH3CN/H2O=75/25 Flow rate : 1.0mL/min Detector : Shodex RI Column temp. : 30°C
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