Durability Against Alkaline Solvents (NH2P-50 4E)

Since the packing material of Asahipak NH2P-50 column is polymer, it is durable against alkaline solvents. To the contrary, silica-based amino columns are not durable against alkaline solvents.

Testing procedureScroll
(1) Equilibrium (1 h)
Eluent100 mM Ammonium acetate (pH9.3)
Flow rate0.1 mL/min
Column temp.Room temp.
(2) Analysis
 
(3) Testing (160 h)
Eluent5 mM NaOH (pH11.4)/CH3CN=25/75
Flow rate0.5 mL/min
Column temp.30 ℃
(4) Replacement (0.5 h)
Eluent100 mM Ammonium acetate (pH9.3)
Flow rate0.1 mL/min
Column temp.Room temp.
(5) Equilibrium (1 h)
Eluent100 mM Ammonium acetate (pH9.3)
Flow rate0.5 mL/min
Column temp.Room temp.
(6) Analysis
 
 
Comparison between before and after testingScroll
No. Saccharides Before testing After testing
Elution volume (mL) Plate number Elution volume (mL) Plate number
1 Fructose   7.52 7100   7.62 8100
2 Glucose   9.63 6100   9.68 7700
3 Sucrose 13.41 8400 13.40 9700
4 Maltose 16.16 7200 16.08 7400
Column
Shodex Asahipak NH2P-50 4E (4.6 mm I.D. x 250 mm)
Eluent
CH3CN/H2O=75/25
Flow rate
:1.0  mL/min
Detector
RI
Column temp.
30 ℃

Sample Name Index

Operation Manual / Certificate of Analysis

Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.

Product Name Index

Applications

Applications (Related Information)