Shodex™ Capture the Essence
Durability Against Alkaline Solvents
Since the packing material of Asahipak NH2P-50 column is polymer, it is durable against alkaline solvents. To the contrary, silica-based amino columns are not durable against alkaline solvents.
Testing procedure
| (1)Equilibrium(1hr) Conditions before testing Eluent : 100mM Ammonium acetate(pH9.3) Flow rate : 0.1mL/min Column temp. : Room temp. |
(2)Analysis |
| (3)Testing(160hrs) Eluent : 5mM NaOH(pH11.4)/CH3CN=25/75 Flow rate : 0.1mL/min Column temp. : 30°C |
(4)Replacement(0.5hr)
Eluent : H2O Flow rate : 0.5mL/min Column temp. : Room temp. |
| (5)Replacement(0.5hr)
Eluent : H2O Flow rate : 0.5mL/min Column temp. : Room temp. |
(6)Analysis |
| Comparison between before and after testing | ||||
|---|---|---|---|---|
| Sample | Before testing | After testing | ||
| Elution volume (mL) | Plate number | Elution volume (mL) | Plate number | |
| 1.Fructose | 7.52 | 7100 | 7.62 | 8100 |
| 2.Glucose | 9.63 | 6100 | 9.68 | 7700 |
| 3.Sucrose | 13.41 | 8400 | 13.40 | 9700 |
| 4.Maltose | 16.16 | 7200 | 16.08 | 7400 |
Column : Shodex Asahipak NH2P-50 4E (4.6mmI.D. x 250mm) Eluent : CH3CN/H2O=75/25 Flow rate : 1.0mL/min Detector : Shodex RI Column temp. : 30°C
Sample name index
Product name index
Applications
- Elution Volume of Saccharides
- Elution Volume of Saccharides (VG-50 4E)
- Separation of Anomer
- Comparison of Amino Column with Amide Column
- Comparison of Columns with Different Counter Ions
- Combination of Columns with Different Counter Ions
- Effects of Free Base Ratio of Amino Group on Plate Number
- Effects of Acetonitrile Concentration on Elution Time (NH2P-50)
- Effects of Acetonitrile Concentration on Elution Time (SZ5532)
- Effects of Acetonitrile Concentration on Plate Number
- Durability Against Acidic Solvents
- Durability Against Eluent Composition Change
- Effects of Sample Solvent Composition
- Effects of Sample Injection Volume
- Effects of Flow Rate (SC1011)
- Effects of Temperature (1)
- Effects of Temperature (2)
- Calibration Curves for Saccharides (NH2P-50)
- Calibration Curves for Saccharides (SZ5532)
- Comparison of NH2P with Silica-based Amino Column (1)
- Comparison of NH2P with Silica-based Amino Column (2)
- Monosaccharides (1) (NH2P-50 4E)
- Monosaccharides (2) (NH2P-50 4E)
- Monosaccharides and Disaccharides (1) (SP0810)
- Monosaccharides and Disaccharides (2) (NH2P-50 4E)
- Monosaccharides and Disaccharides (3) (NH2P-50 4E)
- Mono-, Di- and Trisaccharides (1) (NH2P-50 4E)
- Saccharides in Agricultural Products
- Saccharides in Wood (1) (SP0810)
- Saccharides in Wood (2) (SP0810)
- Palatinose in Food (SC1011)
- Worcester Sauce
- Yogurt: Sugar added (SP0810)
- Yogurt: Sugar added (NH2P-50)
- Roast Sweet Potato (NH2P-50 4E)
- Roast Sweet Potato (SP0810)
- Soybean Flour (SP0810)
- Milk Coffee (NH2P-50 4E)
- Jelly (NH2P-50 4E)
- Saccharides in Bread (KS-801)
- Raspberry Jam
- Candy
- Lactic acid Beverage and Fermented Milk
- Orange and Melon
- Orange Juice
- Chocolate Cake (NH2P-50)
- Chocolate Cake (SZ5532)
- Maltose and Isomaltose
- Saccharide Analysis Using Semi-micro Column
- Saccharides in powdered Milk
- Glucose Derivatives
- Extract of Wheat Rod
- Effects of Organic Solvent in Eluent
- Saccharides in Food
- Monosaccharides Composing Sugar Chain
- Maltose and Nigerose
- Analysis of Ketohexose (SP0810)
- Monosaccharides and Disaccharides (4)
- Cello-oligosaccharides and Ethanol
- Analysis of Rare Sugar (1) (SP0810)
- Galactinol (SC1011)
- Analysis of Rare Sugar (2) (VG-50 4E)
- Saccharides Separation in Presence of Na Salt (DC-613)
- Heptulose Separation (KS-801)
- Saccharides Analysis using Corona Charged Aerosol Detector
- Comparison between NH2P-40 3E and its Conventional Type (NH2P-50 4E)
- Analysis of Saccharides and Furfurals (KS-801)
- Saccharides Analysis in Ionic Liquid (DC-613)
- Gulose (SP0810)
- Analysis of Trehalose According to JP Method (KS-801)
- Simultaneous Analysis of Lactose, Epilactose, and Lactulose (VG-50 4E)
- Analysis of Trehalose According to USP-NF Method (KS-801)
- Saccharides in Wood (3) (NH2P-50 4E)
- Monosaccharides and Disaccharides (5) (VG-50 4E)
- Analysis of Aldoses (VG-50 4E)
- Abnormal Behavior of Chromatogram of Saccharides Using a Pb Type Column for Ligand Exchange Chromatography
- Analysis of Fructose Glucose Syrup According to Japanese Pharmaceutical Excipients Method (KS-801)
- Analysis of Maltose Starch Powder According to Japanese Pharmaceutical Excipients Method (KS-801)
- Analysis of Ribose According to USP-NF Method (KS-801)
- Analysis of Dextrose According to USP-NF Method (SC1011)
- Analysis of Tagatose According to USP-NF Method (SC1011)
- Analysis of Ethyl α-D-Glucoside in Sake (VG-50 4E)
- Analysis of Maltose According to USP-NF Method (KS-801)
- Analysis of Maltose Hydrate According to JP Method (KS-801)
- Analysis of Lactulose According to JP Method (SP0810)
- Analysis of Erythrose and Threose (SC1011)
Applications (related information)
Saccharides and Organic Acids
- Monosaccharides and Oligosaccharides
- Oligosaccharides
- Acid Saccharides, Phosphorylated Saccharides
- Saccharides, Sugar Alcohols, Amino Sugars and Amino Acids
- Saccharides and Organic Acids
- Polysaccharides
- High-selective Analysis of Saccharides
- Sweeteners
- Organic Acids
- Guidelines for Shodex Column Selection : by Separation Mode
- Guidelines for Shodex Column Selection : by Sample Category
- Guidelines for Shodex Column Selection : by Elution Volume
- Guidelines for Shodex Column Selection : by Kinds of Saccharides
- Guidelines for Shodex Column Selection : by Analytical Conditions
- Guidelines for Shodex Column Selection : by Combination of Saccharides
- Explanation on Ligand Exchange Mode
- Guidelines for Shodex Column Selection : Organic Acids
Shodex GLOBAL Website