According to Japanese Pharmaceutical Excipients 2018 method (PSB Notification No. 0328-1 by the Pharmaceutical Safety Bureau, MHLW, dated March 28, 2024 (New instructions)), the assay for fructose glucose syrup (high fructose syrup) is carried out with a column filled with strong acid ion-exchange resin and meets following requirements. The use of SUGAR KS-801 column confirmed the requirements were met.

Resolution between fructose and glucose for a 10-µL injection of the standard solution: ≥ 1.5
Relative standard deviation of each fructose and glucose peak area for the six repeated analysis: ≤ 1.0

*The version at the time of the application acquisition.

Sample: 10 μL
(Standard solution)

1. 1.Glucose 60 mg/mL
2. 2.Fructose 84 mg/mL