Analysis of Fructose Glucose Syrup According to Japanese Pharmaceutical Excipients Method (KS-801)

Fructose glucose syrup was analyzed on SUGAR KS-801 according to Japanese Pharmaceutical Excipients 2013 method. It is necessary for the choice of column to satisfy resolution of fructose/glucose of ≥ 1.5 and relative standard deviation (RSD) of six times repeated measurements of ≤ 1.0 %. It was confirmed that the all conditions were satisfied when they were analyzed using KS-801.

Sample : 10 µL
1. Glucose 60 mg/mL
2. Fructose 84 mg/mL

Column       : Shodex SUGAR KS-801 (8.0 mm I.D. x 300 mm)
Eluent       : H2O
Flow rate    : 0.9 mL/min
Detector     : RI
Column temp. : 50 °C

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