Effects of sample solvent composition when Asahipak NH2P-50 is used is shown. When making sample solution to be injected, sample should be dissolved in a solvent whose composition is as close as the eluent, preferably in the eluent. When 75 % acetonitrile solvent is used for the eluent and a sample cannot be dissolved in it, first, the sample should be dissolved in a solvent which dissolves the sample, and then, adjust the solvent composition to be acetonitrile aqueous solution of 50 % or more.


Sample : 1 mg/mL each, 20 μL
1. meso-Erythritol 2. Xylitol 3. Fructose 4. Glucose 5. Sucrose 6. Lactose 7. Maltose

Column       : Shodex Asahipak NH2P-50 4E (4.6 mm I.D. x 250 mm)
Eluent       : CH3CN/H2O=75/25
Flow rate    : 1.0 mL/min
Detector     : RI
Column temp. : 25 °C