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Effects of Sample Solvent Composition

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Effects of sample solvent composition when Asahipak NH2P-50 is used is shown. When making sample solution to be injected, sample should be dissolved in a solvent whose composition is as close as the eluent, preferably in the eluent. When 75% acetonitrile solvent is used for the eluent and a sample cannot be dissolved in it, first, the sample should be dissolved in a solvent which dissolves the sample, and then, adjust the solvent composition to be acetonitrile aqueous solution of 50% or more.


Sample : 1mg/mL each, 20μL
1. meso-Erythritol 2. Xylitol 3. Fructose 4. Glucose 5. Sucrose 6. Lactose 7. Maltose


Column       : Shodex Asahipak NH2P-50 4E (4.6mmI.D. x 250mm)
Eluent       : CH3CN/H2O=75/25
Flow rate    : 1.0mL/min
Detector     : Shodex RI
Column temp. : 25°C

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