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Mono-, Di- and Trisaccharides (1) (NH2P-50 4E)


Asahipak NH2P-50 is a column for the analysis of saccharides by normal phase mode. Under this mode, saccharides are basically eluted in increasing order of molecular weights. An increase of organic solvent in the eluent delays the elution of saccharides. NH2P column allows the separation of saccharides at room temperature without causing anomer separation as these columns are alkaline environment due to the bonded amino groups.

Sample : 0.5 % each, 10 µL
1. Rhamnose
2. Fructose
3. Glucose
4. Sucrose
5. Maltose
6. Raffinose

Column       : Shodex Asahipak NH2P-50 4E (4.6 mm I.D. x 250 mm)
Eluent       : H2O/CH3CN=25/75
Flow rate    : 1.0 mL/min
Detector     : RI
Column temp. : 30 °C

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