Extract of wheat rod were analyzed using Asahipak NH2P-50 (a column for saccharides analysis).
Sample :
I. Glycerol
F. Fructose
G. Glucose
S. Sucrose
K. Kestose
N. Nystose
DP3-7. Expected polymerization degree of Fructan
Columns : Shodex Asahipak NH2P-50G 4A (4.6mmI.D. x 10mm) + NH2P-50 4E (4.6mmI.D. x 250mm) Eluent : 50mM Ammonium acetate/CH3CN=30/70 Flow rate : 1.0mL/min Detector : Shodex RI Column temp. : 40°C
Courtesy of Dr. Tsuchiya, Ibaraki University
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