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Effects of Acetonitrile Concentration on Elution Time (SZ5532)

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The base material of the packing material of SUGAR SZ5532 is the gel developed for adsorption & partition mode. It is highly cross-linked and its swelling and shrinkage is very small. The counter ion is Zn2+. Zn2+ forms a complex with saccharides but, when the counter ion is Zn2+, saccharides are not so strongly reteined by ligand exchange mode. And, when organic solvents are added in the eluent, saccharides and sugar alcohols are reteined by adsorption & partition mode and their elution volumes become larger by inceasing organic solvent concentration.


Sample :
Rhamnose
meso-Erythritol
Glucose
Xylose
Sucrose
Sorbitol
Isomaltose
myo-Inositol
Maltotriose
Raffinose


Column       : Shodex SUGAR SZ5532 (6.0mmI.D. x 150mm)
Eluent       : CH3CN/H2O 
Flow rate    : 0.6mL/min
Detector     : Shodex RI
Column temp. : 60°C

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