Below table summarizes elution volumes of saccharides using HILICpak VG-25 4D, a polymer-based amino column. Elution values shown are the peak top positions, please contact us to request a chromatogram if you would like to see their elution patterns.
Click here for a list of “Elution Volume of Saccharides” using other sugar analysis columns.
Click here for a list of “Elution Volume of Saccharides” using HILICpak VG-50 4E.
Scroll
| Substance | Elution Volume (mL) | ||
|---|---|---|---|
| Eluent 1 | Eluent 2 | Eluent 3 | |
| Arabinose | 6.70 | 5.43 | 5.66 |
| Arabitol |
7.27 |
5.69 | 6.54 |
| meso-Erythritol | 5.92 | 4.96 | 4.54 |
| Fructose | 7.40 | 5.77 | 6.94 |
| Fucose | 5.85 | 4.89 | 4.76 |
| Galactose | 9.14 | 6.69 | 9.47 |
| Gentiobiose | 19.61 | 10.79 | 41.47 |
| Glucose | 9.23 | 6.71 | 10.56 |
| myo-Inositol | 16.14 | 10.08 | 21.15 |
| Isomaltose | 19.37 | 10.72 | 44.50 |
| Isomaltotriose | 45.14 | 18.63 | N/A |
| Lactitol | 17.75 | 10.13 | 29.79 |
| Lactose | 16.23 | 9.57 | 26.83 |
| Lactulose | 13.36 | 8.31 | 19.23 |
| Maltitol | 16.15 | 9.47 | 30.86 |
| Maltose | 16.40 | 9.65 | 34.19 |
| Maltotriose | 31.91 | 14.83 | N/A |
| Mannitol | 9.12 | 6.62 | 10.29 |
| Mannose | 8.04 | 6.28 | 9.22 |
| Melezitose | 25.52 | 12.55 | N/A |
| Melibiose | 20.09 | 11.07 | 39.98 |
| Palatinit | 18.78 | 10.42 | 2 peaks |
| Palatinose | 14.17 | 8.61 | 25.86 |
| Rhamnose | 5.50 | 4.68 | 4.42 |
| Ribose | 5.63 | 4.80 | 4.46 |
| Sorbitol | 8.81 | 6.48 | 8.98 |
| Sorbose | 7.89 | 6.05 | 7.56 |
| Sucrose | 13.38 | 8.31 | 22.83 |
| Talose | 6.68 | 5.37 | 6.27 |
| Trehalose | 18.87 | 10.44 | 51.17 |
| Trehalulose | 14.99 | 8.96 | 28.34 |
| Xylitol | 7.16 | 5.64 | 6.09 |
| Xylobiose | 9.50 | 6.69 | 10.55 |
| Xylose | 6.72 | 5.42 | 6.03 |
- N/A
- ：未確認
- Column
- ：Shodex HILICpak VG-25 4D (4.6 mm I.D. x 150 mm)
- Eluent
- ：(1) H2O/CH3CN=20/80
(2) H2O/CH3CN=25/75
(3) H2O/CH3OH/CH3CN=10/5/85 - Flow rate
- ：(Eluent 1 and 2) 0.6 mL/min
(Eluent 3) 1.0 mL/min - Detector
- ：RI
- Column temp.
- ：(Eluent 1 and 2) 40 ℃
(Eluent 3) 50 ℃
Sample Name Index
- meso-Erythritol
- myo-Inositol
- Arabinose
- Arabitol
- Fructose
- Fucose
- Galactose
- Gentiobiose
- Glucose
- Isomaltose
- Isomaltotriose
- Lactitol
- Lactose
- Lactulose
- Maltitol
- Maltose
- Maltotriose
- Mannitol
- Mannose
- Melezitose
- Melibiose
- Palatinit, Isomalt
- Palatinose, Isomaltulose
- Rhamnose
- Ribose
- Sorbitol, Glucitol
- Sorbose
- Sucrose, Saccharose
- Talose
- Trehalose
- Trehalulose
- Xylitol
- Xylobiose
- Xylose
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Elution Volume of Saccharides
- Elution Volume of Saccharides (VG-50 4E)
- Separation of Anomer
- Comparison of Amino Column with Amide Column
- Comparison of Columns with Different Counter Ions
- Combination of Columns with Different Counter Ions
- Effects of Free Base Ratio of Amino Group on Plate Number (NH2P-50 4E)
- Effects of Acetonitrile Concentration on Elution Time (NH2P-50 4E)
- Effects of Acetonitrile Concentration on Elution Time (SZ5532)
- Effects of Acetonitrile Concentration on Plate Number (NH2P-50 4E)
- Durability Against Acidic Solvents (NH2P-50 4E)
- Durability Against Alkaline Solvents (NH2P-50 4E)
- Durability Against Eluent Composition Change (NH2P-50 4E)
- Effects of Sample Solvent Composition (NH2P-50 4E)
- Effects of Sample Injection Volume (NH2P-50 4E)
- Effects of Flow Rate (SC1011)
- Effects of Temperature (1) (SC1011)
- Effects of Temperature (2)
- Calibration Curves for Saccharides (NH2P-50 4E)
- Calibration Curves for Saccharides (SZ5532)
- Comparison of NH2P with Silica-based Amino Column (1)
- Comparison of NH2P with Silica-based Amino Column (2)
- Monosaccharides (1) (NH2P-50 4E)
- Monosaccharides (2) (NH2P-50 4E)
- Monosaccharides and Disaccharides (1) (SP0810)
- Monosaccharides and Disaccharides (2) (NH2P-50 4E)
- Monosaccharides and Disaccharides (3) (NH2P-50 4E)
- Monosaccharides and Disaccharides (4) (SZ5532)
- Monosaccharides and Disaccharides (5) (VG-50 4E)
- Monosaccharides and Disaccharides (8) (VG-25 4D)
- Mono-, Di- and Trisaccharides (1) (NH2P-50 4E)
- Saccharides in Agricultural Products (SC1011)
- Saccharides in Wood (1) (SP0810)
- Saccharides in Wood (2) (SP0810)
- Saccharides in Wood (3) (NH2P-50 4E)
- Palatinose in Food (SC1011)
- Worcester Sauce (KS-801)
- Yogurt: Sugar added (SP0810)
- Yogurt: Sugar added (NH2P-50 4E)
- Roast Sweet Potato (NH2P-50 4E)
- Roast Sweet Potato (SP0810)
- Soybean Flour (SP0810)
- Milk Coffee (NH2P-50 4E)
- Jelly (NH2P-50 4E)
- Saccharides in Bread (KS-801)
- Lactic acid Beverage and Fermented Milk (SH1011)
- Orange and Melon (SH1011)
- Orange Juice (SC1011)
- Chocolate Cake (NH2P-50 4E)
- Chocolate Cake (SZ5532)
- Maltose and Isomaltose (SZ5532)
- Saccharide Analysis Using Semi-micro Column (NH2P-50 2D)
- High Sensitive Analysis of Saccharides in powdered Milk (NH2P-50 4E)
- Glucose Derivatives (NH2P-50 4E)
- Extract of Wheat Rod (NH2P-50 4E)
- Effects of Organic Solvent in Eluent (NH2P-50 4E)
- Saccharides in Food (SC1011)
- Monosaccharides Composing Sugar Chain (SH1011)
- Maltose and Nigerose (NH2P-50 4E)
- Analysis of Ketohexose (SP0810)
- Cello-oligosaccharides and Ethanol (KS-802)
- Analysis of Rare Sugar (1) (SP0810)
- Analysis of Rare Sugar (2) (VG-50 4E)
- Galactinol (SC1011)
- Saccharides Separation in Presence of Na Salt (DC-613)
- Heptulose Separation (KS-801)
- Saccharides Analysis using Corona Charged Aerosol Detector (NH2P-50 4E)
- Comparison between NH2P-40 3E and its Conventional Type (NH2P-50 4E)
- Analysis of Saccharides and Furfurals (KS-801)
- Saccharides Analysis in Ionic Liquid (DC-613)
- Gulose (SP0810)
- Simultaneous Analysis of Lactose, Epilactose, and Lactulose (VG-50 4E)
- Simultaneous Analysis of Lactose, Epilactose, and Lactulose (2) (VG-25 4D)
- Analysis of Aldoses (VG-50 4E)
- Abnormal Behavior of Chromatogram of Saccharides Using a Pb Type Column for Ligand Exchange Chromatography
- Analysis of Fructose Glucose Syrup (High Fructose Syrup) According to Japanese Pharmaceutical Excipients Method (KS-801)
- Analysis of Maltose Starch Powder According to Japanese Pharmaceutical Excipients Method (KS-801)
- Analysis of Ribose According to USP-NF Method (KS-801)
- Analysis of Dextrose According to USP-NF Method (SC1011)
- Analysis of Tagatose According to USP-NF Method (SC1011)
- Analysis of Maltose According to USP-NF Method (KS-801)
- Analysis of Trehalose According to USP-NF Method (KS-801)
- Analysis of Maltose Hydrate According to JP Method (KS-801)
- Analysis of Trehalose According to JP Method (KS-801)
- Analysis of Lactulose According to JP Method (SP0810)
- Analysis of L-Rhamnose in Accordance with the Japan’s Specifications and Standards for Food Additives (NH2P-50 4D)
- Analysis of Ethyl α-D-Glucoside in Sake (VG-50 4E)
- Analysis of Erythrose and Threose (SC1011)
- Analysis of 2-Deoxy-D-glucose (SC1011)
- Analysis of 2-Deoxy-D-glucose (VG-50 4E)
- Analysis of Dextrose and Fructose in Cranberry Juice (NH2P-50 4E)
- Simultaneous Analysis of 15 Components Including Monosaccharides, Disaccharides, and Oligosaccharides by HILIC Mode (VG-50 4E)