Elution Volume of Saccharides (VG-25 4D)

Below table summarizes elution volumes of saccharides using HILICpak VG-25 4D, a polymer-based amino column. Elution values shown are the peak top positions, please contact us to request a chromatogram if you would like to see their elution patterns.

Click here for a list of “Elution Volume of Saccharides” using other sugar analysis columns.
Click here for a list of “Elution Volume of Saccharides” using HILICpak VG-50 4E.

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Substance Elution Volume (mL)
Eluent 1 Eluent 2 Eluent 3
Arabinose   6.70 5.43 5.66
Arabitol

  7.27

5.69 6.54
meso-Erythritol   5.92 4.96 4.54
Fructose   7.40 5.77 6.94
Fucose   5.85 4.89 4.76
Galactose   9.14 6.69 9.47
Gentiobiose 19.61 10.79 41.47
Glucose   9.23 6.71 10.56
myo-Inositol 16.14 10.08 21.15
Isomaltose 19.37 10.72 44.50
Isomaltotriose 45.14 18.63 N/A
Lactitol 17.75 10.13 29.79
Lactose 16.23 9.57 26.83
Lactulose 13.36 8.31 19.23
Maltitol 16.15 9.47 30.86
Maltose 16.40 9.65 34.19
Maltotriose 31.91 14.83 N/A
Mannitol   9.12 6.62 10.29
Mannose   8.04 6.28 9.22
Melezitose 25.52 12.55 N/A
Melibiose 20.09 11.07 39.98
Palatinit 18.78 10.42 2 peaks
Palatinose 14.17 8.61 25.86
Rhamnose   5.50 4.68 4.42
Ribose   5.63 4.80 4.46
Sorbitol   8.81 6.48 8.98
Sorbose   7.89 6.05 7.56
Sucrose 13.38 8.31 22.83
Talose   6.68 5.37 6.27
Trehalose 18.87 10.44 51.17
Trehalulose 14.99 8.96 28.34
Xylitol   7.16 5.64 6.09
Xylobiose   9.50 6.69 10.55
Xylose   6.72 5.42 6.03
N/A
未確認
Column
Shodex HILICpak VG-25 4D (4.6 mm I.D. x 150 mm)
Eluent
(1) H2O/CH3CN=20/80
(2) H2O/CH3CN=25/75
(3) H2O/CH3OH/CH3CN=10/5/85
Flow rate
(Eluent 1 and 2) 0.6 mL/min
(Eluent 3) 1.0 mL/min
Detector
RI
Column temp.
(Eluent 1 and 2) 40 ℃
(Eluent 3) 50 ℃

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