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Durability Against Eluent Composition Change

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Since the swelling and shrinking of the packing material of Asahipak NH2P-50 columns is considerably small even when the eluent composition is changed, any mixing ratio of acetonitrile and water can be used with the NH2P-50 columns.




Comparison between before and after testing
SampleBefore testingAfter testing
Elution volume
(mL)
Plate number Elution volume
(mL)
Plate number
1. Fructose 7.61 11600 7.56 9800
2. Glucose 9.80 7500 9.72 6200
3. Sucrose 13.86 11700 13.75 9300
4. Maltose 16.78 8300 16.66 6700
Column       : Shodex Asahipak NH2P-50 4E (4.6mmI.D. x 250mm)
Eluent       : CH3CN/H2O=75/25
Flow rate    : 1.0mL/min
Detector     : Shodex RI
Column temp. : 30°C

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