Durability Against Eluent Composition Change (NH2P-50 4E)

Since the swelling and shrinking of the packing material of Asahipak NH2P-50 columns is considerably small even when the eluent composition is changed, any mixing ratio of acetonitrile and water can be used with the NH2P-50 columns.

 
Comparison between before and after testingScroll
No. Saccharides Before testing After testing
Elution volume (mL) Plate number Elution volume (mL) Plate number
1 Fructose   7.61 11600   7.56 9800
2 Glucose   9.80   7500   9.72 6200
3 Sucrose 13.86 11700 13.75 9300
4 Maltose 16.78   8300 16.66 6700
Column
Shodex Asahipak NH2P-50 4E (4.6 mm I.D. x 250 mm)
Eluent
CH3CN/H2O=75/25
Flow rate
:1.0  mL/min
Detector
RI
Column temp.
30 ℃

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