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Analysis of Dextrose According to USP-NF Method (SC1011)


According to the United States Pharmacopeia and the National Formulary (USP 40-NF 35*), the assay of dextrose (D-glucose) and the analysis of related substances should be carried out with a column with L19 packing material and meets following requirements. The SUGAR SC1011 confirmed the requirements were met.

System suitability requirements:
    Resolution of maltotriose and maltos (in sample solution A): ≥ 1.3

*The version at the time of the application acquisition. 

Sample : 20 μL
System suitability solution : Maltotriose, Maltose, Fructose 0.1 mg/mL each (in H2O)
Sample solution A : Dextrose (D-Glucose) 30 mg/mL(in H2O)
1. Maltotriose, 2. Maltose, 3. Dextrose, 4. Fructose

Column       : Shodex SUGAR SC1011 (8.0 mm I.D. x 300 mm)
Eluent       : H2O
Flow rate    : 0.3 mL/min
Detector     : RI
Column temp. : 85 °C

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