Analysis of 2-Deoxy-D-glucose (VG-50 4E)

2-Deoxy-D-glucose is a glucose analog which has the 2-hydroxyl group replaced by hydrogen, and it is not metabolized by glycolysis. 2-Deoxy-D-glucose is synthesized from D-Glucal, so both compounds may be present during the synthesis. HILICpak VG-50 4E, a polymer-based amino column, is used to analyze 2-Deoxy-D-glucose, D-Glucal, and glucose.

Sample : 10 µL
0.15 %(w/v) each in H2O/CH3CN=5/5
1. D-Glucal
2. 2-Deoxy-D-glucose
3. Glucose

Column       : Shodex HILICpak VG-50 4E (4.6 mm I.D. x 250 mm)
Eluent       : H2O/CH3CN=12/88
Flow rate    : 1.0 mL/min
Detector     : RI
Column temp. : 40 °C

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