Orange and Melon (SH1011)

Saccharides in orange and melon were analyzed using SUGAR SH1011. Besides saccharides, some unknown peaks were observed though organic acids and pigments were eliminated by the mini-column.

(the pretreatment of fruit juice)
First, the sample was filtrated using a 0.45 μm disposable filter.
Then, it was treated with a mini-column packed with anion exchanger. And, it was filtrated again.

Sample :
Mandarin orange, 2.5 μL
Musk melon, 2 μL
1. Glucose
2. Fructose

Columns      : Shodex SUGAR SH-G (6.0 mm I.D. x 50 mm) + SH1011 (8.0 mm I.D. x 300 mm)
Eluent       : 5 mM H2SO4 aq.
Flow rate    : 1.0 mL/min
Detector     : RI, UV (210 nm)
Column temp. : 50 °C

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