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Orange and Melon


Saccharides in orange and melon were analyzed using SUGAR SH1011. Besides saccharides, some unknown peaks were observed though organic acids and pigments were eliminated by the mini-column.

(the pretreatment of fruit juice)
First, the sample was filtrated using a 0.45μm disposable filter.
Then, it was treated with a mini-column packed with anion exchanger. And, it was filtrated again.

Sample :
Mandarin orange, 2.5μL
Musk melon, 2μL
1. Glucose
2. Fructose

Columns      : Shodex SUGAR SH-G (6.0mmI.D. x 50mm) + SH1011 (8.0mmI.D. x 300mm)
Eluent       : 5mM H2SO4 aq.
Flow rate    : 1.0mL/min
Detector     : Shodex RI, UV(210nm)
Column temp. : 50°C

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