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Saccharides Separation in Presence of Na Salt (DC-613)


RSpak DC-613 column is for the separation of sugars using ligand-exchange and normal-phase modes. The ligand used is Na, thus even for the samples containing sodium salt does not require desalting sample preparation steps. The samples treated with acids can be analyzed after neutralizing with sodium hydroxide.
*DC-613 sugar analysis requires mobile phase with high concentration of ACN, thus samples containing a large amount of salt may cause precipitation.

Sample : 10μL, 0.1% each
1. Xylose
2. Sucrose
3. Cellobiose
4. Lactose

Column       : Shodex RSpak DC-613 (6.0mmI.D. x 150mm)
Eluent       : CH3CN/H2O=70/30
Flow rate    : 0.8mL/min
Detector     : Shodex RI
Column temp. : 50°C

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