Monosaccharides Composing Sugar Chain (SH1011)

Monosaccharides which compose sugar chain are separated using SUGAR SH1011. Glycoproteins and glycolipids, which are exist in animals and palnts, are composed of protein and lipid molecles combined with sugar chains. And, sugar chains are composed of monosaccharides such as D-glucose, D-mannose, L-fucose, N-acetylglucosamine, N-acetylgalactosamine and sialic acids. N-Acetylneuraminic acid is the most popular sialic acid.


Sample : 10 μL
1. N-Acetylneuraminic acid, Sialic acid 0.125 mg/mL
2. Glucose 1.25 mg/mL
3. Mannose 1.25 mg/mL
4. Fucose 1.25 mg/mL
5. N-Acetyl-D-glucosamine 0.125 mg/mL
6. N-Acetyl-D-galactosamine 0.125 mg/mL


Column       : Shodex SUGAR SH1011 (8.0 mm I.D. x 300 mm)
Eluent       : 5 mM H2SO4 aq.
Flow rate    : 0.6 mL/min
Detector     : (above); UV (210 nm)  
               (below); RI         
Column temp. : 60 °C

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