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Monosaccharides Composing Sugar Chain


Monosaccharides which compose sugar chain are separated using SUGAR SH1011. Glycoproteins and glycolipids, which are exist in animals and palnts, are composed of protein and lipid molecles combined with sugar chains. And, sugar chains are composed of monosaccharides such as D-glucose, D-mannose, L-fucose, N-acetylglucosamine, N-acetylgalactosamine and sialic acids. N-Acetylneuraminic acid is the most popular sialic acid.

Sample : 10μL
1. N-Acetylneuraminic acid, Sialic acid 0.125mg/mL
2. Glucose 1.25mg/mL
3. Mannose 1.25mg/mL
4. Fucose 1.25mg/mL
5. N-Acetyl-D-glucosamine 0.125mg/mL
6. N-Acetyl-D-galactosamine 0.125mg/mL

Column       : Shodex SUGAR SH1011 (8.0mmI.D. x 300mm)
Eluent       : 0.005M H2SO4 aq.
Flow rate    : 0.6mL/min
Detector     : (above); UV(210nm)  
               (below); Shodex RI         
Column temp. : 60°C

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