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Comparison of Amino Column with Amide Column


It has been reported that the anomer separation does not take place under strong alkaline conditions. Amino columns such as Asahipak NH2P-50 4E allow the separation of saccharides without causing the anomer separation as they can be used under alkaline conditions at room temperature. (Please refer to Prevention of Anomer Separation.)
Amide columns are also used for the separation of saccharides. However, acryl-amide goups are not alkaline, it is necessary to perform the separation at elevated temperature to prevent anomer separation.

Sample : 5 mg/mL each, 10 μL
1. Fructose
2. Glucose
3. Sucrose
4. Maltose



Column       : Shodex Asahipak NH2P-50 4E, Amide column from other manufacturer
               (4.6 mm I.D. x 250 mm each)
Eluent       : CH3CN/H2O=75/25
Flow rate    : 1.0 mL/min
Detector     : RI
Column temp. : 30 °C

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