Comparison of NH2P with Silica-based Amino Column (2)

Compared with the case when Asahipak NH2P columns are used, when silica-based amino columns are used, detection sensitivity and plate number of saccharides are different according to the type of saccharides. And, the plate number of some specific saccharide such as galactose is extremely low.


Sample : 20 μL
1. 2 % Galactose
2. 1 % Sucrose
3. 1 % Maltose

Columns      : Shodex Asahipak NH2P-50 4E (4.6 mm I.D. x 250 mm),
               Silica based amino column (other manufacturer)
Eluent       : CH3CN/H2O=75/25
Flow rate    : 1.0 mL/min
Detector     : RI
Column temp. : 25 °C