Polymer-based amino column, Asahipak NH2P-50 4E with corona charged aerosol detector was used to analyze saccharides in wood. Baseline separation can be obtained using a gradient method.


Sample : 0.025 % each, 10 μL
1. Arabinose
2. Xylose
3. Mannose
4. Galactose
5. Glucose
6. Cellobiose

Column       : Shodex Asahipak NH2P-50 4E (4.6 mm I.D. x 250 mm)
Eluent       : (A); CH3CN　(B); H2O 
               82 % (A)(0 to 15 min), 70 % (A)(15 to 23 min), 82 % (A)(23 to 28 min）
Flow rate    : 1.0 mL/min
Detector     : Corona charged aerosol
Column temp. : 30 °C