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Separation of Anomer


It has been reported that the anomer separation does not take place at high temperature. Therefore, when using SUGAR series (SP0810, SC1011, KS-801, SZ5532 or SC1211 ), set up the column temperature at 70-80 °C.
Please refer to Prevention of Anomer Separation.

Sample : 0.5% each, 10μL
Glucose, Mannose, Xylose, Galactose, Arabinose

Column       : Shodex SUGAR SC1011 (8.0mmI.D. x 300mm)
Eluent       : H2O
Flow rate    : 0.7mL/min
Detector     : Shodex RI
Column temp. : 29°C, 70°C 

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