It has been reported that the anomer separation does not take place at high temperature. Therefore, when using SUGAR series (SP0810, SC1011, KS-801, SZ5532 or SC1211 ), set up the column temperature at 70-80 °C.
Please refer to Prevention of Anomer Separation.

Sample : 0.5 % each, 10 μL
Glucose, Mannose, Xylose, Galactose, Arabinose

Column       : Shodex SUGAR SC1011 (8.0 mm I.D. x 300 mm)
Eluent       : H2O
Flow rate    : 0.7 mL/min
Detector     : RI
Column temp. : 29 °C, 70 °C 

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