Mono- and di-saccharides in ionic liquid were analyzed by RSpak DC-613. Saccharides were separated under ligand-exchange and HILIC separation modes. The presence of one type of ionic liquids, 1-Ethyl-3-methylimidazolium chloride, did not interfere with saccharide separation.
Sample : 0.1% each, 10μL
1. Xylose
2. Sucrose
3. Cellobiose
4. Lactose
※Sample solution :
1% 1-Ethyl-3-methylimidazolium Chloride/CH3CN=50/50
Column : Shodex RSpak DC-613 (6.0mmI.D. x 150mm) Eluent : CH3CN/H2O=70/30 Flow rate : 0.8mL/min Detector : Shodex RI Column temp. : 50°C
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