Analysis of Sorbitol in Nicardipine Hydrochloride Injection (NH2P-50 4E)

Nicardipine hydrochloride injection is used for hypertensive emergencies and acute heart failure. The United States Pharmacopeia and the National Formulary (USP-NF 2022 ISSUE 2 *) uses a silica-based amino column to measure the sorbitol content in nicardipine hydrochloride injection. In this application, Asahiak NH2P-50 4E, a polymer-based amino column, was used to analyze sorbitol using the USP monograph as a reference. The result confirmed the feasibility of NH2P-50 4E for the sorbitol analysis.

*The version at the time of the application acquisition.

Sample: 25 µL

  1. 1.Sorbitol 4.8 mg/mL
Chromatogram of Sorbitol
Shodex Asahipak NH2P-50 4E (4.6 mm I.D. x 250 mm)
CH3CN and 1 g/L of Tetrabutylammonium hydrogen sulfate in water (70:30)
Flow rate
1.0 mL/min
RI (50 ℃)
Column temp.
40 ℃

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