Two organic sulfonic acids, taurine and taurocholic acid, were analyzed by LC/MS/MS using a HILIC mode column HILCpak VT-50 2D. It is confirmed that the acids can be detected down to the level of ng/mL.
Sample : 5 μL
0.01 µM each
2. Taurocholic acid
Column : Shodex HILICpak VT-50 2D (2.0 mm I.D. x 150 mm) Eluent : 50 mM HCOONH4 aq./CH3CN=20/80 Flow rate : 0.3 mL/min Detector : ESI-MS/MS (MRM Negative)
Column temp. : 30 °C
Sample Name Index
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