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LC/MS/MS Analysis of Organic Sulfonic Acids (VT-50 2D)


Two organic sulfonic acids, taurine and taurocholic acid, were analyzed by LC/MS/MS using a HILIC mode column HILCpak VT-50 2D. It is confirmed that the acids can be detected down to the level of ng/mL.

Sample : 5 μL
0.01 µM each
(in H2O/CH3CN=1/3)
1. Taurine
2. Taurocholic acid

Column       : Shodex HILICpak VT-50 2D (2.0mmI.D. x 150mm)
Eluent       : 50mM HCOONH4 aq./CH3CN=20/80
Flow rate    : 0.3mL/min
Detector     : ESI-MS/MS (MRM Negative)
Column temp. : 30°C

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