Two organic sulfonic acids, taurine and taurocholic acid, were analyzed by LC/MS/MS using a HILIC mode column HILCpak VT-50 2D. It is confirmed that the acids can be detected down to the level of ng/mL.


Sample : 5 μL
0.01 µM each
(in H₂O/CH₃CN=1/3)
1. Taurine
2. Taurocholic acid

Column       : Shodex HILICpak VT-50 2D (2.0 mm I.D. x 150 mm)
Eluent       : 50 mM HCOONH4 aq./CH3CN=20/80
Flow rate    : 0.3 mL/min
Detector     : ESI-MS/MS (MRM Negative)
Column temp. : 30 °C