High polar compounds, such as metformin (an oral antidiabetic drug in biguanide class) are not retained by reversed-phase chromatography. Instead, HILIC mode is suitable for the analysis of polar compounds. A polymer-based amino column, Asahipak NH2P-40 2D, was used in the LC/MS method. Eluent containing a high acetonitrile ratio provides a high sensitive MS detection.
*Sales of Asahipak NH2P-40 2D used in this application is discontinued. Please consider trying Asahipak NH2P-50 2D.

Sample: 5 μL

1. 1.Metformin-HCl 5 ng/mL (in H₂O/CH₃CN=5/95)