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LC/MS Analysis of Metformin (NH2P-40 2D)


High polar compounds, such as metformin (an oral antidiabetic drug in biguanide class) are not retained by reversed-phase chromatography. Instead, HILIC mode is suitable for the analysis of polar compounds. A polymer-based amino column, Asahipak NH2P-40 2D, was used in the LC/MS method. Eluent containing a high acetonitrile ratio provides a high sensitive MS detection.

Sample : 5μL
1. Metformin -HCl 5ng/mL
(in H2O/CH3CN=5/95)

Column       : Shodex Asahipak NH2P-40 2D (2.0mmI.D. x 150mm)
Eluent       : 50mM HCOONH4 aq./CH3CN=5/95
Flow rate    : 0.2mL/min
Detector     : ESI-MS (SIM)
Column temp. : 30°C

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