High polar compounds, such as metformin (an oral antidiabetic drug in biguanide class) are not retained by reversed-phase chromatography. Instead, HILIC mode is suitable for the analysis of polar compounds. A polymer-based amino column, Asahipak NH2P-40 2D, was used in the LC/MS method. Eluent containing a high acetonitrile ratio provides a high sensitive MS detection.
*Sales of the product used in this application is discontinued, however if you are still interested in this application, please contact us.
Sample : 5 μL
1. Metformin -HCl 5 ng/mL
Column : Shodex Asahipak NH2P-40 2D (2.0 mm I.D. x 150 mm) Eluent : 50 mM HCOONH4 aq./CH3CN=5/95 Flow rate : 0.2 mL/min Detector : ESI-MS (SIM) Column temp. : 30 °C