High polar compounds, such as metformin (an oral antidiabetic drug in biguanide class) are not retained by reversed-phase chromatography. Instead, HILIC mode is suitable for the analysis of polar compounds. A polymer-based amino column, Asahipak NH2P-40 2D, was used in the LC/MS method. Eluent containing a high acetonitrile ratio provides a high sensitive MS detection.
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Sample : 5 μL
1. Metformin -HCl 5 ng/mL
(in H₂O/CH₃CN=5/95)

Column       : Shodex Asahipak NH2P-40 2D (2.0 mm I.D. x 150 mm)
Eluent       : 50 mM HCOONH4 aq./CH3CN=5/95
Flow rate    : 0.2 mL/min
Detector     : ESI-MS (SIM)
Column temp. : 30 °C