When using an alkaline eluent with a higher pH than the pKa sample value, basic drug components which have tertiary amino groups can be retained by the column because the amino groups are not dissociated and, in consequence, the hydrophilicity of the drug components decrease. Such analysis conditions can be realized for Asahipak ODP-50 columns because they can be used at pH11.
Sample : 1. Benzocaine, 2. Lidocaine, 3. Tetracaine hydrochloride
Column : Shodex Asahipak ODP-50 4D (4.6 mm I.D. x 150 mm) Eluent : 25 mM Phosphate buffer/CH3CN=60/40 Flow rate : 0.6 mL/min Detector : UV (254 nm) Column temp. : 30 °C
Sample Name Index
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.