LC/MS/MS Analysis of Citicoline (VT-50 2D)

Citicoline, a therapeutic agent for disorders of consciousness and pancreatitis was analyzed using using HILICpak VT-50 2D, a polymer-based HILIC mode column, with LC/MS/MS detection.

Sample: 5 μL

  1. 1.Citicoline sodium 10 ng/mL (in H2O/CH3CN=1/1)
Column
Shodex HILICpak VT-50 2D (2.0 mm I.D. x 150 mm)
Eluent
25 mM HCOONH4 aq./CH3CN=50/50
Flow rate
0.2 mL/min
Detector
ESI-MS/MS (MRM Positive)
Column temp.
40 ℃

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