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LC/MS/MS Analysis of Citicoline (VT-50 2D)


Citicoline, a therapeutic agent for disorders of consciousness and pancreatitis was analyzed using using HILICpak VT-50 2D, a polymer-based HILIC mode column, with LC/MS/MS detection.

Sample : 5µL
1. Citicoline sodium 10ng/mL
(in H2O/CH3CN=1/1)

Column       : Shodex HILICpak VT-50 2D (2.0mmI.D. x 150mm)
Eluent       : 25mM HCOONH4 aq./CH3CN=50/50
Flow rate    : 0.2mL/min
Detector     : ESI-MS/MS (MRM Positive)
Column temp. : 40°C

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