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Scopolamine and Atropine

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Scopolamine and atropine were analyzed using Asahipak ODP-50 4D (a column for reversed phase chromatography).
Please refer to Analysis of Short Amines without Using Ion Pair Reagent .


Sample :
1. Scopolamine
2. Atropine


Column       : Shodex Asahipak ODP-50 4D (4.6mmI.D. x 150mm)
Eluent       : 50mM Phosphate buffer(pH10)/CH3CN=70/30
Flow rate    : 0.6mL/min
Detector     : UV(210nm)
Column temp. : 30°C
Column : ODS (other manufacturer 4.6mmI.D. x 150mm) Eluent : 0.1% Sodium 1-pentanesulfonate/CH3CN=60/40 Flow rate : 1.4mL/min Detector : UV(210nm) Column temp. : 60°C

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