​Because of its anti-inflammatory effects, allantoin is used in eye-drops and disinfectants. In this application, HILICpak VG-50 4E, a polymer-base amino column, was used to analyze allantoin under HILIC mode. A highly linear calibration curve was obtained in the concentration range 1 to 1,000 µg/mL. Allantoin was detected in a commercial disinfectant without the influence of other components. The recovery rate against the amount mentioned in the product’s ingredient table was 95 %. This demonstrated the method’s feasibility for the analysis of commercial products.

Sample preparation

1. 1.Measure 20 mg of a commercial disinfectant. Add 8 mL of H₂O/CH₃CN=1/1 and mix well. Measure up to 10 mL with H₂O/CH₃CN=1/1.
2. 2.Filter using 0.45-μm filter and use the filtrate for injection.

Sample: 5 μL
(Standard solution) 0.1 mg/mL (in H₂O/CH₃CN=1/1)
(Commercial disinfectant)

1. 1.Allantoin