Analysis of α-GPC in Supplements (NH2P-50 4E)

α-GPC (L-α-glyceryl phosphoryl choline) is a naturally occurring choline derivative. It is a precursor of acetylcholine that acts on parasympathetic nerves and have a potential for treating Alzheimer's disease by improving the brain function.
In this application, a commercial supplement containing α-GPC was analyzed by a polymer-based amino column, Asahipak NH2P-50 4E using its HILIC mode. It provided a good linear calibration curve in the concentration range 0.05 to 1 mg/L. The recovery of the α-GPC was almost 100 %, compared to the manufacturer’s claimed value.

(Sample Preparation)
1) Add 5 mL of eluent to 10 mg of sample and sonicate for 10 minutes
2) Filter using a 0.45-µm membrane filter and use it as the injection sample.

Sample : 20 μL
Standard 1 mg/mL (in eluent)
1. α-GPC

Column       : Shodex Asahipak NH2P-50 4E (4.6 mm I.D. x 250 mm)
Eluent       : 50 mM NaH2PO4 aq./CH3CN=40/60
Flow rate    : 1.0 mL/min
Detector     : RI
Column temp. : 40 °C

Sample Name Index

Operation Manual / Certificate of Analysis

Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.

Product Name Index


Applications (Related Information)