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Analysis of α-GPC in Supplements (NH2P-50 4E)


α-GPC (L-α-glyceryl phosphoryl choline) is a type of naturally occurring choline derivative.
It is a precursor of acetylcholine that acts on parasympathetic nerves,
It is believed to have potential as a brain function improving agent for treating Alzheimer's disease.
Polymer-based amino column α-GPC was measured in HILIC mode using Asahipak NH2P-50 4E.
A highly linear calibration curve is obtained in the concentration range of 0.05 to 1 mg/mL.
An analysis of a commercial supplement as a real sample confirmed that the recovery rate is almost 100 %.

(Method of preparing sample solution of commercially available supplement)
(1) Add 5 mL of eluent to 10 mg of sample and sonicate for 10 minutes
(2) Filter the supernatant with a 0.45 μm filter

Sample : 20 μL
Standard 1 mg/mL (in eluent)
1. α-GPC

Column       : Shodex Asahipak NH2P-50 4E (4.6 mm I.D. x 250 mm)
Eluent       : 50 mM NaH2PO4 aq./CH3CN=40/60
Flow rate    : 1.0 mL/min
Detector     : Shodex RI
Column temp. : 40 °C

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